The use of transcriptional profiles to predict adult mosquito age under field conditions

被引:86
作者
Cook, Peter E.
Hugo, Leon E.
Iturbe-Ormaetxe, Inaki
Williams, Craig R.
Chenoweth, Stephen F.
Ritchie, Scott A.
Ryan, Peter A.
Kay, Brian H.
Blows, Mark W.
O'Neill, Scott L. [1 ]
机构
[1] Univ Queensland, Sch Integrat Biol, Brisbane, Qld 4072, Australia
[2] Queensland Inst Med Res, Herston, Qld 4029, Australia
[3] James Cook Univ N Queensland, Anton Breinl Ctr Publ Hlth Trop Med, Cairns, Qld 4870, Australia
[4] Queensland Hlth, Trop Populat Hlth Unit, Cairns, Qld 4870, Australia
关键词
Aedes aegypti; age-grading; gene expression; cuticular hydrocarbons; multivariate calibration;
D O I
10.1073/pnas.0604875103
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Age is a critical determinant of an adult female mosquito's ability to transmit a range of human pathogens. Despite its central importance, relatively few methods exist with which to accurately determine chronological age of field-caught mosquitoes. This fact is a major constraint on our ability to fully understand the relative importance of vector longevity to disease transmission in different ecological contexts. It also limits our ability to evaluate novel disease control strategies that specifically target mosquito longevity. We report the development of a transcriptional profiling approach to determine age of adult female Aedes aegypti under field conditions. We demonstrate that this approach surpasses current cuticular hydrocarbon methods for both accuracy of predicted age as well as the upper limits at which age can be reliably predicted. The method is based on genes that display age-dependent expression in a range of dipteran insects and, as such, is likely to be broadly applicable to other disease vectors.
引用
收藏
页码:18060 / 18065
页数:6
相关论文
共 35 条
[1]  
[Anonymous], 2003, STAT ANAL COMPOSITIO
[2]  
[Anonymous], WHO MONOGRAPH
[3]   Gene expression during the life cycle of Drosophila melanogaster [J].
Arbeitman, MN ;
Furlong, EEM ;
Imam, F ;
Johnson, E ;
Null, BH ;
Baker, BS ;
Krasnow, MA ;
Scott, MP ;
Davis, RW ;
White, KP .
SCIENCE, 2002, 297 (5590) :2270-2275
[4]   Fungal pathogen reduces potential for malaria transmission [J].
Blanford, S ;
Chan, BHK ;
Jenkins, N ;
Sim, D ;
Turner, RJ ;
Read, AF ;
Thomas, MB .
SCIENCE, 2005, 308 (5728) :1638-1641
[5]   The potential of virulent Wolbachia to modulate disease transmission by insects [J].
Brownstin, JS ;
Hett, E ;
O'Neill, SL .
JOURNAL OF INVERTEBRATE PATHOLOGY, 2003, 84 (01) :24-29
[6]   Aedes aegypti (Diptera: Culicidae) age determination by cuticular hydrocarbon analysis of female legs [J].
Desena, ML ;
Edman, JD ;
Clark, JM ;
Symington, SB ;
Scott, TW .
JOURNAL OF MEDICAL ENTOMOLOGY, 1999, 36 (06) :824-830
[7]   THE ANALYSIS OF PARASITE TRANSMISSION BY BLOODSUCKING INSECTS [J].
DYE, C .
ANNUAL REVIEW OF ENTOMOLOGY, 1992, 37 :1-19
[8]   Field validation of Aedes aegypti (Diptera: Culicidae) age estimation by analysis of cuticular hydrocarbons [J].
Gerade, BB ;
Lee, SH ;
Scott, TW ;
Edman, JD ;
Harrington, LC ;
Kitthawee, S ;
Jones, JW ;
Clark, JM .
JOURNAL OF MEDICAL ENTOMOLOGY, 2004, 41 (02) :231-238
[9]   Age-grading adult insects: a review of techniques [J].
Hayes, EJ ;
Wall, R .
PHYSIOLOGICAL ENTOMOLOGY, 1999, 24 (01) :1-10
[10]   The genome sequence of the malaria mosquito Anopheles gambiae [J].
Holt, RA ;
Subramanian, GM ;
Halpern, A ;
Sutton, GG ;
Charlab, R ;
Nusskern, DR ;
Wincker, P ;
Clark, AG ;
Ribeiro, JMC ;
Wides, R ;
Salzberg, SL ;
Loftus, B ;
Yandell, M ;
Majoros, WH ;
Rusch, DB ;
Lai, ZW ;
Kraft, CL ;
Abril, JF ;
Anthouard, V ;
Arensburger, P ;
Atkinson, PW ;
Baden, H ;
de Berardinis, V ;
Baldwin, D ;
Benes, V ;
Biedler, J ;
Blass, C ;
Bolanos, R ;
Boscus, D ;
Barnstead, M ;
Cai, S ;
Center, A ;
Chatuverdi, K ;
Christophides, GK ;
Chrystal, MA ;
Clamp, M ;
Cravchik, A ;
Curwen, V ;
Dana, A ;
Delcher, A ;
Dew, I ;
Evans, CA ;
Flanigan, M ;
Grundschober-Freimoser, A ;
Friedli, L ;
Gu, ZP ;
Guan, P ;
Guigo, R ;
Hillenmeyer, ME ;
Hladun, SL .
SCIENCE, 2002, 298 (5591) :129-+