Naringin Protects against Rotenone-induced Apoptosis in Human Neuroblastoma SH-SY5Y Cells

被引：81

作者：

Kim, Hak-Jae ^{[1
]}

Song, Jeong Yoon ^{[2
]}

Park, Hae Jeong ^{[1
]}

Park, Hyun-Kyung ^{[2
]}

Yun, Dong Hwan ^{[3
]}

Chung, Joo-Ho ^{[1
]}

机构：

[1] Kyung Hee Univ, Sch Med, Dept Pharmacol, Kohwang Med Res Inst, Seoul 130701, South Korea

[2] Kyung Hee Univ, Sch Med, EW Neo Med Ctr, Seoul 130701, South Korea

[3] Kyung Hee Univ, Sch Med, Dept Phys Med & Rehabil, Seoul 130701, South Korea

来源：

KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY | 2009年 / 13卷 / 04期

关键词：

Apoptosis; Naringin; Parkinson's disease; Rotenone; SH-SY5Y; NEURONS IN-VIVO; PARKINSONS-DISEASE; CYTOCHROME-C; MAP KINASES; COMPLEX-I; ACTIVATION; FLAVONOIDS; INHIBITOR; JNK; MECHANISM;

D O I：

10.4196/kjpp.2009.13.4.281

中图分类号：

R9 [药学];

学科分类号：

100702 [药剂学];

摘要：

Rotenone, a mitochondrial complex I inhibitor, can induce the pathological features of Parkinson's disease (PD). In the present study, naringin, a grapefruit flavonoid, inhibited rotenone-induced cell death in human neuroblastoma SH-SY5Y cells. We assessed cell death and apoptosis by measuring mitogen-activated protein kinase (MAPKs) and caspase (CASPs) activities and by performing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, 4,6-diamidino-2-phenylindole (DAPI) staining, and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining. Naringin also blocked rotenone-induced phosphorylation of Jun NH2-terminal protein kinase (JNK) and P38, and prevented changes in B-cell CLL/lymphoma 2 (BCL2) and BCL2-associated X protein (BAX) expression levels. In addition, naringin reduced the enzyme activity of caspase 3 and cleavages of caspase 9, poly (ADP-ribose) polymerase (PARP), and caspase 3. These results suggest that naringin has a neuroprotective effect on rotenone-induced cell death in human neuroblastoma SH-SY5Y cells.

引用

页码：281 / 285

页数：5

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