Genome-wide Analysis of RNA Polymerase II Termination at Protein-Coding Genes

被引：92

作者：

Baejen, Carlo ^{[1
]}

Andreani, Jessica ^{[1
,5
]}

Torkler, Phillipp ^{[1
]}

Battaglia, Sofia ^{[1
]}

Schwalb, Bjoern ^{[1
]}

Lidschreiber, Michael ^{[4
]}

Maier, Kerstin C. ^{[1
]}

Boltendahl, Andrea ^{[1
]}

Rus, Petra ^{[1
]}

Esslinger, Stephanie ^{[2
,3
]}

Soding, Johannes ^{[1
]}

Cramer, Patrick ^{[1
]}

机构：

[1] Max Planck Inst Biophys Chem, Am Fassberg 11, D-37077 Gottingen, Germany

[2] Ludwig Maximilians Univ Munchen, Gene Ctr Munich, Feodor Lynen Str 25, D-81377 Munich, Germany

[3] Ludwig Maximilians Univ Munchen, Dept Biochem, Feodor Lynen Str 25, D-81377 Munich, Germany

[4] Karolinska Inst, Ctr Innovat Med & Sci Life Lab, Dept Biochem & Nutr, Novum Halsovagen 7, S-14183 Huddinge, Sweden

[5] Univ Paris Sud, Univ Paris Saclay, CNRS, CEA,Inst Integrat Biol Cell, F-91198 Gif Sur Yvette, France

来源：

MOLECULAR CELL | 2017年 / 66卷 / 01期

基金：

欧洲研究理事会;

关键词：

PROMOTES TRANSCRIPTION TERMINATION; MESSENGER-RNA; PROCESSING FACTORS; ELONGATION RATE; NONCODING RNA; YEAST; CLEAVAGE; END; REVEALS; DOMAIN;

D O I：

10.1016/j.molcel.2017.02.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

At the end of protein-coding genes, RNA polymerase (Pol) II undergoes a concerted transition that involves 3'-processing of the pre-mRNA and transcription termination. Here, we present a genome-wide analysis of the 3'-transition in budding yeast. We find that the 3'-transition globally requires the Pol II elongation factor Spt5 and factors involved in the recognition of the polyadenylation (pA) site and in endonucleolytic RNA cleavage. Pol II release from DNA occurs in a narrow termination window downstream of thepAsite and requires the "torpedo'' exonuclease Rat1 (XRN2 in human). The Rat1-interacting factor Rai1 contributes to RNA degradation downstream of the pA site. Defects in the 3'-transition can result in increased transcription at downstream genes.

引用

页码：38 / +

页数：18

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