A hydrogen-producing, hydrogenase-free mutant strain of Nostoc punctiforme ATCC 29133

被引：58

作者：

Lindberg, P ^{[1
]}

Schütz, K

Happe, T

Lindblad, P

机构：

[1] Uppsala Univ, EBC, Dept Physiol Bot, SE-75236 Uppsala, Sweden

[2] Univ Bonn, Inst Bot, D-53115 Bonn, Germany

来源：

INTERNATIONAL JOURNAL OF HYDROGEN ENERGY | 2002年 / 27卷 / 11-12期

关键词：

Nostoc; uptake hydrogenase mutant; nitrogenase; hydrogen evolution;

D O I：

10.1016/S0360-3199(02)00121-0

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The hupL gene, encoding the uptake hydrogenase large subunit, in Nostoc sp. strain ATCC 29133, a strain lacking a bidirectional hydrogenase, was inactivated by insertional mutagenesis. Recombinant strains were isolated and analysed, and one hupL(-) strain, NHM5, was selected for further study. Cultures of NHM5 were grown under nitrogen-fixing conditions and H-2 evolution under air was observed using an H-2 electrode. (C) 2002 International Association for Hydrogen Energy. Published by Elsevier Science Ltd. All rights reserved.

引用

页码：1291 / 1296

页数：6

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