Differential gene expression between human schwannoma and control Schwann cells

被引:34
作者
Hanemann, C. O.
Bartelt-Kirbach, B.
Diebold, R.
Kaempchen, K.
Langmesser, S.
Utermark, T.
机构
[1] Peninsula Med Sch, Inst Biomed & Clin Sci, Plymouth PL6 8BU, Devon, England
[2] Univ Ulm, Dept Heurol, Clin Res Ctr, Ulm, Germany
[3] Univ Fribourg, Inst Biochim, CH-1700 Fribourg, Switzerland
[4] Dana Farber Canc Inst, Dept Canc Biol, Boston, MA 02115 USA
关键词
cells cultured; gene expression; human; neurofibromatosis; Schwann cells; schwannoma;
D O I
10.1111/j.1365-2990.2006.00769.x
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
The NF2 gene encodes the tumour suppressor protein merlin. The mutation of a single allele of this gene causes the autosomal dominantly inherited disease neurofibromatosis type 2 (NF2), which is characterized mainly by vestibular schwannoma carrying a second hit mutation. Complete lack of merlin is also found in spontaneous schwannomas and meningiomas. As the events leading to schwannoma development are largely unknown we investigated the differences in gene expression between schwannoma cells from NF2 patients and normal human primary Schwann cells by cDNA array analysis. We identified 41 genes whose expression levels differed by more than factor 2. Most of these clones were corroborated by real-time reverse transcription polymerase chain reaction analysis. By this method a total of seven genes with increased and seven genes with decreased mRNA levels in schwannoma compared with normal Schwann cells could be identified. Regulated clones, some of which not been described in Schwann cells earlier, included matrix metalloproteinase's, growth factors, growth factor receptors and tyrosine kinases.
引用
收藏
页码:605 / 614
页数:10
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