A function for AAMP in Nod2-mediated NF-κB activation

被引：37

作者：

Bielig, H. ^{[1
]}

Zurek, B. ^{[1
]}

Kutsch, A. ^{[1
]}

Menning, M. ^{[1
]}

Philpott, D. J. ^{[2
]}

Sansonetti, P. J. ^{[3
,4
]}

Kufer, T. A. ^{[1
]}

机构：

[1] Univ Cologne, Inst Med Microbiol Immunol & Hyg, D-50931 Cologne, Germany

[2] Univ Toronto, Dept Immunol, Toronto, ON, Canada

[3] Inst Pasteur, Unite Patogenie Microbienne Mol, Paris, France

[4] Inst Pasteur, INSERM 786, Paris, France

来源：

MOLECULAR IMMUNOLOGY | 2009年 / 46卷 / 13期

关键词：

Signalling; NLR; CARD15; NF-kappa B; INTESTINAL EPITHELIAL-CELLS; GENE-EXPRESSION; PROTEIN; INNATE; IMMUNOGLOBULIN; RECEPTORS; DOMAINS; ERBIN; NOD2;

D O I：

10.1016/j.molimm.2009.04.022

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The WD40 repeat containing angio-associated migratory cell protein (AAMP) was identified as a new binding partner of the human nucleotide-binding domain, leucine rich repeat containing (NLR) family member Nod2 in a yeast two-hybrid screen. Co-immunoprecipitations from human cells verified this interaction and revealed that an internal peptide of AAMP spanning three WD40 domains was sufficient for this interaction. AAMP was found to be ubiquitously expressed in different human cell-lines and exhibited a predominant cytosolic localization in epithelial cells. Functionally, using overexpression and siRNA knock-down, we showed that AAMP modulates Nod2- and Nod1-mediated NF-kappa B activation in HEK293T cells. Taken together, our data support a new function of AAMP in regulating innate immune responses initiated by the NLR protein Nod2. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：2647 / 2654

页数：8

共 24 条

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[3]

Membrane recruitment of NOD2 in intestinal epithelial cells is essential for nuclear factor-κB activation in muramyl dipeptide recognition [J].