TRB3 modulates C2C12 differentiation by interfering with Akt activation

被引：47

作者：

Kato, Satomi ^{[1
]}

Du, Keyong ^{[1
]}

机构：

[1] Tufts Univ, New England Med Ctr, Mol Oncol Res Inst, Boston, MA 02111 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 353卷 / 04期

关键词：

Akt; Tribbles; TRB3; differentiation; muscles; C2C12; MyoD; myogenin;

D O I：

10.1016/j.bbrc.2006.12.161

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

TRB3 is a member of TRB protein family characterized by containing a variant kinase domain without enzymatic activity. Interacting with Ser/Thr protein kinases Akt, TRB3 impairs Akt activation induced by growth factors and insulin. In this study we have examined the potential role of TRB3 in muscle differentiation. Our data indicated that the expression of TRB3 is downregulated during C2C12 cells undergoing muscle differentiation and that overexpression of TRB3 inhibits Akt activation during differentiation. Correspondingly, overexpression of TRB3 inhibits, while knockdown TRB3 enhances C2C12 differentiation. Thus, our studies indicated that TRB3 plays a critical role in muscle differentiation. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：933 / 938

页数：6

共 27 条

[1] Growth factors controlling muscle development [J].

Bass, J ;

Oldham, J ;

Sharma, M ;

Kambadur, R .

DOMESTIC ANIMAL ENDOCRINOLOGY, 1999, 17 (2-3) :191-197

[2] Emerging roles of pseudokinases [J].

Boudeau, Jerome ;

Miranda-Saavedra, Diego ;

Barton, Geoffrey J. ;

Alessi, Dario R. .

TRENDS IN CELL BIOLOGY, 2006, 16 (09) :443-452

[3] SKIP3, a novel Drosophila tribbles ortholog, is overexpressed in human tumors and is regulated by hypoxia [J].

Bowers, AJ ;

Scully, S ;

Boylan, JF .

ONCOGENE, 2003, 22 (18) :2823-2835

[4] TRB3:: A tribbles homolog that inhibits Akt/PKB activation by insulin in liver [J].

Du, KY ;

Herzig, S ;

Kulkarni, RN ;

Montminy, M .

SCIENCE, 2003, 300 (5625) :1574-1577

[5]

FLORINI JR, 1993, ADV EXP MED BIOL, V343, P319

[6] Tribbles: A family of kinase-like proteins with potent signalling regulatory function [J].

Hegedus, Z. ;

Czibula, A. ;

Kiss-Toth, E. .

CELLULAR SIGNALLING, 2007, 19 (02) :238-250

[7] Akt-phosphorylation of p300 at Ser-1834 is essential for its histone acetyltransferase and transcriptional activity [J].

Huang, WC ;

Chen, CC .

MOLECULAR AND CELLULAR BIOLOGY, 2005, 25 (15) :6592-6602

[8] Lentiviral vectors: Regulated gene expression [J].

Kafri, T ;

van Praag, H ;

Gage, FH ;

Verma, IM .

MOLECULAR THERAPY, 2000, 1 (06) :516-521

[9] Human tribbles, a protein family controlling mitogen-activated protein kinase cascades [J].

Kiss-Toth, E ;

Bagstaff, SM ;

Sung, HY ;

Jozsa, V ;

Dempsey, C ;

Caunt, JC ;

Oxley, KM ;

Wyllie, DH ;

Polgar, T ;

Harte, M ;

O'Neill, LAJ ;

Qwarnstrom, EE ;

Dower, SK .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (41) :42703-42708

[10] Skeletal muscle-selective knockout of LKB1 increases insulin sensitivity, improves, glucose homeostasis, and decreases TRB3 [J].

Koh, Ho-Jin ;

Arnolds, David E. ;

Fujii, Nobuharu ;

Tran, Thien T. ;

Rogers, Marc J. ;

Jessen, Niels ;

Li, Yangfeng ;

Liew, Chong Wee ;

Ho, Richard C. ;

Hirshman, Michael F. ;

Kulkarni, Rohit N. ;

Kahn, C. Ronald ;

Goodyear, Laurie J. .

MOLECULAR AND CELLULAR BIOLOGY, 2006, 26 (22) :8217-8227

← 1 2 3 →