Three human RNA polymerase III-specific subunits form a subcomplex with a selective function in specific transcription Initiation

被引:132
作者
Wang, ZX [1 ]
Roeder, RG [1 ]
机构
[1] ROCKEFELLER UNIV,BIOCHEM & MOL BIOL LAB,NEW YORK,NY 10021
关键词
transcription; RNA polymerase III; transcription initiation;
D O I
10.1101/gad.11.10.1315
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Transcription by RNA polymerase III involves recruitment of the polymerase by template-bound accessory factors, followed by initiation, elongation, and termination steps. An immunopurification approach has been used to demonstrate that human RNA Pol III is composed of 16 subunits, some of which are apparently modified in HeLa cells. Partial denaturing conditions and sucrose gradient sedimentation at high salt result in the dissociation of a subcomplex that includes hRPC32, hRPC39, and hRPC62. Cognate cDNAs were isolated and shown to encode three subunits that are specific to RNA Pol III and homologous to three yeast subunits. The human RNA Pol III core lacking the subcomplex functions in transcription elongation and termination following nonspecific initiation on a tailed template, but fails to show promoter-dependent transcription initiation in conjunction with accessory factors. The capability for specific transcription initiation can be restored either by the natural subcomplex or by a stable subcomplex composed of recombinant hRPC32, hRPC39, and hRPC62 polypeptides. One component (hRPC39) of this subcomplex interacts physically with both hTBP and hTFIIIB90, two subunits of human RNA Pol III transcription initiation factor IIIB. These data strongly suggest that the hRPC32-hRPC39-hRPC62 subcomplex directs RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and hRPC39.
引用
收藏
页码:1315 / 1326
页数:12
相关论文
共 39 条
[1]   ORIENTATION AND TOPOGRAPHY OF RNA POLYMERASE-III IN TRANSCRIPTION COMPLEXES [J].
BARTHOLOMEW, B ;
DURKOVICH, D ;
KASSAVETIS, GA ;
GEIDUSCHEK, EP .
MOLECULAR AND CELLULAR BIOLOGY, 1993, 13 (02) :942-952
[2]   TFIIIC RELIEVES REPRESSION OF U6 SNRNA TRANSCRIPTION BY CHROMATIN [J].
BURNOL, AF ;
MARGOTTIN, F ;
HUET, J ;
ALMOUZNI, G ;
PRIOLEAU, MN ;
MECHALI, M ;
SENTENAC, A .
NATURE, 1993, 362 (6419) :475-477
[3]   UNIQUE TATA-BINDING PROTEIN-CONTAINING COMPLEXES AND COFACTORS INVOLVED IN TRANSCRIPTION BY RNA POLYMERASE-II AND POLYMERASE-III [J].
CHIANG, CM ;
GE, H ;
WANG, ZX ;
HOFFMANN, A ;
ROEDER, RG .
EMBO JOURNAL, 1993, 12 (07) :2749-2762
[4]   RPC82 ENCODES THE HIGHLY CONSERVED, 3RD-LARGEST SUBUNIT OF RNA POLYMERASE-C (III) FROM SACCHAROMYCES-CEREVISIAE [J].
CHIANNILKULCHAI, N ;
STALDER, R ;
RIVA, M ;
CARLES, C ;
WERNER, M ;
SENTENAC, A .
MOLECULAR AND CELLULAR BIOLOGY, 1992, 12 (10) :4433-4440
[5]   A UNIVERSALLY CONSERVED REGION OF THE LARGEST SUBUNIT PARTICIPATES IN THE ACTIVE-SITE OF RNA-POLYMERASE-III [J].
DIECI, G ;
HERMANNLEDENMAT, S ;
LUKHTANOV, E ;
THURIAUX, P ;
WERNER, M ;
SENTENAC, A .
EMBO JOURNAL, 1995, 14 (15) :3766-3776
[6]   ACCURATE TRANSCRIPTION INITIATION BY RNA POLYMERASE-II IN A SOLUBLE EXTRACT FROM ISOLATED MAMMALIAN NUCLEI [J].
DIGNAM, JD ;
LEBOVITZ, RM ;
ROEDER, RG .
NUCLEIC ACIDS RESEARCH, 1983, 11 (05) :1475-1489
[7]  
EDWARDS AM, 1991, J BIOL CHEM, V266, P71
[8]   ROLE OF A SMALL RNA POL-II SUBUNIT IN TATA TO TRANSCRIPTION START SITE SPACING [J].
FURTERGRAVES, EM ;
HALL, BD ;
FURTER, R .
NUCLEIC ACIDS RESEARCH, 1994, 22 (23) :4932-4936
[9]   RNA POLYMERASE-III(C) AND ITS TRANSCRIPTION FACTORS [J].
GABRIELSEN, OS ;
SENTENAC, A .
TRENDS IN BIOCHEMICAL SCIENCES, 1991, 16 (11) :412-416
[10]   RNA-POLYMERASE-II SUBUNIT RPB9 IS REQUIRED FOR ACCURATE START SITE SELECTION [J].
HULL, MW ;
MCKUNE, K ;
WOYCHIK, NA .
GENES & DEVELOPMENT, 1995, 9 (04) :481-490