Development of a Two-Color Fluorescence In Situ Hybridization Technique for Species-Level Identification of Human-Infectious Cryptosporidium spp.

被引：12

作者：

Alagappan, A. ^{[2
]}

Bergquist, P. L. ^{[2
,3
]}

Ferrari, B. C. ^{[1
]}

机构：

[1] Univ New S Wales, Sch Biotechnol & Biomol Sci, Sydney, NSW 2052, Australia

[2] Macquarie Univ, Dept Chem & Biomol Sci & Environm Biotechnol CRC, Sydney, NSW 2109, Australia

[3] Univ Auckland, Sch Med, Dept Mol Med & Pathol, Auckland, New Zealand

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2009年 / 75卷 / 18期

关键词：

MOLECULAR CHARACTERIZATION; HIV; TRANSMISSION; GENOTYPES; ANIMALS; OOCYSTS; LIMA; PERU;

D O I：

10.1128/AEM.00643-09

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A two-color fluorescence in situ hybridization assay that allows for the simultaneous identification of Cryptosporidium parvum and C. hominis was developed. The assay is a simple, rapid, and cost-effective tool for the detection of the major Cryptosporidium species of concern to public health.

引用

页码：5996 / 5998

页数：3

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