High-speed counter-current chromatography of apple procyanidins

被引：21

作者：

Shibusawa, Y

Yanagida, A

Ito, A

Ichihashi, K

Shindo, H

Ito, Y

机构：

[1] Tokyo Univ Pharm & Life Sci, Sch Pharm, Dept Analyt Chem, Hachioji, Tokyo 1920392, Japan

[2] NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2000年 / 886卷 / 1-2期

关键词：

counter-current chromatography; apples; food analysis; procyanidins; tannins; polyphenols; flavonoids;

D O I：

10.1016/S0021-9673(00)00448-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Apple procyanidins were separated by high-speed counter-current chromatography using a type-J multilayer coil planet centrifuge. Several two-phase solvent systems with a wide range of hydrophobicities from a non-polar hexane system to polar n-butanol systems were evaluated their performance in terms of the partition coefficient and the retention of the phase. The best separation of procyanidins B and C was achieved with a two-phase solvent system composed of n-butanol-methyl tert.-butyl ether-acetonitrile-0.1% trifluoroacetic acid (2:4:3:8) using the lower phase as a mobile at a flow-rate of 1.0 ml/min. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：65 / 73

页数：9

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