ATP-dependent positive supercoiling of DNA by 13S condensin: A biochemical implication for chromosome condensation

被引：319

作者：

Kimura, K

Hirano, T

机构：

[1] Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724

来源：

CELL | 1997年 / 90卷 / 04期

关键词：

D O I：

10.1016/S0092-8674(00)80524-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

13S condensin is a five-subunit protein complex that plays a central role in mitotic chromosome condensation in Xenopus egg extracts. Two core subunits of this complex, XCAP-C and XCAP-E, belong to an emerging family of putative ATPases, the SMC family. We report here that 13S condensin has a DNA-stimulated ATPase activity and exhibits a high affinity for structured DNAs such as cruciform DNA. 13S condensin is able to introduce positive supercoils into a closed circular DNA in the presence of bacterial or eukaryotic topoisomerase I. The supercoiling reaction is ATP-dependent. We propose that 13S condensin wraps DNA in a right-handed direction by utilizing the energy of ATP hydrolysis. This reaction may represent a key mechanism underlying the compaction of chromatin fibers during mitosis.

引用

收藏

页码：625 / 634

页数：10

相关论文

共 48 条

[1]

[Anonymous], 1988, Antibodies: A Laboratory Manual

[2] A 3-DIMENSIONAL APPROACH TO MITOTIC CHROMOSOME STRUCTURE - EVIDENCE FOR A COMPLEX HIERARCHICAL ORGANIZATION [J].

BELMONT, AS ;

SEDAT, JW ;

AGARD, DA .

JOURNAL OF CELL BIOLOGY, 1987, 105 (01) :77-92

[3] Chromatid segregation at anaphase requires the barren product, a novel chromosome-associated protein that interacts with topoisomerase II [J].

Bhat, MA ;

Philp, AV ;

Glover, DM ;

Bellen, HJ .

CELL, 1996, 87 (06) :1103-1114

[4] INTERACTION OF A PROTEIN FROM RAT-LIVER NUCLEI WITH CRUCIFORM DNA [J].

BIANCHI, ME .

EMBO JOURNAL, 1988, 7 (03) :843-849

[5] SPECIFIC RECOGNITION OF CRUCIFORM DNA BY NUCLEAR-PROTEIN HMG1 [J].

BIANCHI, ME ;

BELTRAME, M ;

PAONESSA, G .

SCIENCE, 1989, 243 (4894) :1056-1059

[6] Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii [J].

Bult, CJ ;

White, O ;

Olsen, GJ ;

Zhou, LX ;

Fleischmann, RD ;

Sutton, GG ;

Blake, JA ;

FitzGerald, LM ;

Clayton, RA ;

Gocayne, JD ;

Kerlavage, AR ;

Dougherty, BA ;

Tomb, JF ;

Adams, MD ;

Reich, CI ;

Overbeek, R ;

Kirkness, EF ;

Weinstock, KG ;

Merrick, JM ;

Glodek, A ;

Scott, JL ;

Geoghagen, NSM ;

Weidman, JF ;

Fuhrmann, JL ;

Nguyen, D ;

Utterback, TR ;

Kelley, JM ;

Peterson, JD ;

Sadow, PW ;

Hanna, MC ;

Cotton, MD ;

Roberts, KM ;

Hurst, MA ;

Kaine, BP ;

Borodovsky, M ;

Klenk, HP ;

Fraser, CM ;

Smith, HO ;

Woese, CR ;

Venter, JC .

SCIENCE, 1996, 273 (5278) :1058-1073

[7] DPY-27 - A CHROMOSOME CONDENSATION PROTEIN HOMOLOG THAT REGULATES C. ELEGANS DOSAGE COMPENSATION THROUGH ASSOCIATION WITH THE X-CHROMOSOME [J].

CHUANG, PT ;

ALBERTSON, DG ;

MEYER, BJ .

CELL, 1994, 79 (03) :459-474

[8] Sex-specific assembly of a dosage compensation complex on the nematode X chromosome [J].

Chuang, PT ;

Lieb, JD ;

Meyer, BJ .

SCIENCE, 1996, 274 (5293) :1736-1739

[9] PROTEIN TRACKING-INDUCED SUPERCOILING OF DNA - A TOOL TO REGULATE DNA TRANSACTIONS IN-VIVO [J].

DROGE, P .

BIOESSAYS, 1994, 16 (02) :91-99

[10] Large scale chromosome structure and organization [J].

Earnshaw, William C. .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 1991, 1 (02) :237-244

← 1 2 3 4 5 →