Production and secretion of interleukin-lα proteins by rat testis

被引：13

作者：

Gustafsson, K

Sultana, T

Zetterström, CK

Setchell, BP

Siddiqui, A

Weber, G

Söder, O

机构：

[1] Astrid Lindgren Childrens Hosp, Dept Woman & Child Hlth, Pediat Endocrinol Unit, SE-17176 Stockholm, Sweden

[2] Karolinska Hosp & Inst, Tumor Biochem Unit, Ctr Mol Med, Stockholm, Sweden

[3] Univ Adelaide, Dept Anat Sci, Adelaide, SA 5005, Australia

[4] Aga Khan Univ, Karachi, Pakistan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 297卷 / 03期

关键词：

interleukin-l alpha; testis; paracrine; seminiferous tubules; macrophages;

D O I：

10.1016/S0006-291X(02)02239-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The present study characterizes constitutively expressed rat testicular interleukin-1alpha (IL-1alpha) proteins. IL-1 bioactivity of crude testis protein was completely neutralized by IL-1alpha antiserum, IL-1 receptor antagonist, and soluble type I IL-1 receptor. Upon nondenaturating gel permeation chromatography, bioactive IL-1 eluted at molecular sizes of 45, 3 1, and 17 kDa and at charges of pH 5.7 and 6.0 after chromatofocusing. SDS-PAGE/Western blot analysis of proteins extracted from whole testis, seminiferous tubules, interstitial, and seminiferous tubule fluids all demonstrated IL-1alpha immunoreactivity at 45, 24, and 19kDa. Activated macrophages and tissue proteins from endotoxin treated rats showed inummoreactive 31 and 19kDa IL-1alpha. The results indicate that the testis produces three isoforms of IL-1alpha proteins that are secreted into the interstitial compartment and tubular lumen where they may exert paracrine functions. The testicular IL-1alpha isoforms may represent posttranslationally modified precursor, mature IL-1alpha, and a 24-kDa alternate splice form. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：492 / 497

页数：6

共 27 条

[1] INTERLEUKIN-1-BETA, BUT NOT INTERLEUKIN-1-ALPHA, INDUCES ACUTE INFLAMMATION-LIKE CHANGES IN THE TESTICULAR MICROCIRCULATION OF ADULT-RATS [J].

BERGH, A ;

SODER, O .

JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 1990, 17 (02) :155-165

[2]

BEUSCHER HU, 1988, J BIOL CHEM, V263, P4023

[3] Multifactorial induction of gene expression and nuclear localization of mouse interleukin 1 alpha [J].

Burysek, L ;

Houstek, J .

CYTOKINE, 1996, 8 (06) :460-467

[4] CIRCULATING INTERLEUKIN-1 AND TUMOR NECROSIS FACTOR IN SEPTIC SHOCK AND EXPERIMENTAL ENDOTOXIN FEVER [J].

CANNON, JG ;

TOMPKINS, RG ;

GELFAND, JA ;

MICHIE, HR ;

STANFORD, GG ;

VANDERMEER, JWM ;

ENDRES, S ;

LONNEMANN, G ;

CORSETTI, J ;

CHERNOW, B ;

WILMORE, DW ;

WOLFF, SM ;

BURKE, JF ;

DINARELLO, CA .

JOURNAL OF INFECTIOUS DISEASES, 1990, 161 (01) :79-84

[5]

GIRI JG, 1985, J IMMUNOL, V134, P343

[6] CONSTITUTIVE PRODUCTION OF LYMPHOCYTE ACTIVATING FACTORS BY NORMAL-TISSUES IN THE ADULT-RAT [J].

GRANHOLM, T ;

SODER, O .

JOURNAL OF CELLULAR BIOCHEMISTRY, 1991, 46 (02) :143-151

[7] CYTOKINE RESPONSIVENESS IN GERM-FREE AND CONVENTIONAL NMRI MICE [J].

GRANHOLM, T ;

FROYSA, B ;

LUNDSTROM, C ;

WAHAB, A ;

MIDTVEDT, T ;

SODER, O .

CYTOKINE, 1992, 4 (06) :545-550

[8] ISOLATION AND PARTIAL CHARACTERIZATION OF AN INTERLEUKIN-1-LIKE FACTOR FROM RAT TESTIS INTERSTITIAL FLUID [J].

GUSTAFSSON, K ;

SODER, O ;

POLLANEN, P ;

RITZEN, EM .

JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 1988, 14 (02) :139-150

[9]

HAZUDA DJ, 1988, J BIOL CHEM, V263, P8473

[10]

HAZUDA DJ, 1991, J BIOL CHEM, V266, P7081

← 1 2 3 →