Dynamic behavior of transcription factors on a natural promoter in living cells

被引：167

作者：

Becker, M

Baumann, C

John, S

Walker, DA

Vigneron, M

McNally, JG

Hager, GL

机构：

[1] NCI, Lab Receptor Biol & Gene Express, Ctr Canc Res, NIH, Bethesda, MD 20892 USA

[2] CNRS, ULP ESBS, INSERM, CR1,UMR 7100, F-67400 Illkirch Graffenstaden, France

来源：

EMBO REPORTS | 2002年 / 3卷 / 12期

关键词：

D O I：

10.1093/embo-reports/kvf244

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Through the use of photobleaching techniques, we examined the dynamic interaction of three members of the transcription apparatus with a target promoter in living cells. The glucocorticoid receptor (GR) interacting protein 1 (GRIP-1) exhibits a half maximal time for fluorescent recovery (tau(R)) of 5 s, reflecting the same rapid exchange as observed for GR. In contrast, the large subunit (RPB1) of RNA polymerase II (pol II) required 13 min for complete fluorescence recovery, consistent with its function as a processive enzyme. We also observe a complex induction profile for the kinetics of GR-stimulated transcription. Our results indicate that GR and GRIP-1 as components of the activating complex are in a dynamic equilibrium with the promoter, and must return to the template many times during the course of transcriptional activation.

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页码：1188 / 1194

页数：7

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