A method for detecting sister chromatid exchanges using prematurely condensed chromosomes and immunogold-silver staining

被引:19
作者
Asakawa, Y [1 ]
Gotoh, E [1 ]
机构
[1] NATL INST HLTH,DIV GENET RESOURCES,SHINJUKU KU,TOKYO 162,JAPAN
关键词
D O I
10.1093/mutage/12.3.175
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
We have developed a method for detecting sister chromatid exchanges using premature chromosome condensation and immunogold labelling followed by silver enhancement. Cells were grown in bromodeoxyuridine before sister chromatid exchanges were generated with mitomycin C. Calyculin A was then used to condense the chromosomes prematurely, Finally, incorporated bromodeoxyuridine was detected using a gold-conjugated antibody followed by silver enhancement. Exchanged chromatids could easily be seen in the resulting chromosomes. This simple method allows sister chromatid exchanges to be detected with great sensitivity and resolution.
引用
收藏
页码:175 / 177
页数:3
相关论文
共 17 条
[1]   ANALYSIS OF SISTER CHROMATID EXCHANGE FORMATION INVIVO IN MOUSE SPERMATOGONIA AS A NEW TEST SYSTEM FOR ENVIRONMENTAL MUTAGENS [J].
ALLEN, JW ;
LATT, SA .
NATURE, 1976, 260 (5550) :449-451
[2]   OKADAIC ACID - A NEW PROBE FOR THE STUDY OF CELLULAR-REGULATION [J].
COHEN, P ;
HOLMES, CFB ;
TSUKITANI, Y .
TRENDS IN BIOCHEMICAL SCIENCES, 1990, 15 (03) :98-102
[3]   ANALYSIS OF FREQUENCY AND DISTRIBUTION OF SISTER CHROMATID EXCHANGES IN CULTURED HUMAN LYMPHOCYTES [J].
CROSSEN, PE ;
DRETS, ME ;
ARRIGHI, FE ;
JOHNSTON, DA .
HUMAN GENETICS, 1977, 35 (03) :345-352
[4]   INHIBITION OF PROTEIN-SERINE THREONINE PHOSPHATASES DIRECTLY INDUCES PREMATURE CHROMOSOME CONDENSATION IN MAMMALIAN SOMATIC-CELLS [J].
GOTOH, E ;
ASAKAWA, Y ;
KOSAKA, H .
BIOMEDICAL RESEARCH-TOKYO, 1995, 16 (01) :63-68
[5]  
Gotoh E, 1996, INT J RADIAT BIOL, V70, P517, DOI 10.1080/095530096144716
[6]   USE OF ANTIBODY SPECIFIC FOR BROMODEOXYURIDINE FOR IMMUNOFLUORESCENT DETERMINATION OF DNA-REPLICATION IN SINGLE CELLS AND CHROMOSOMES [J].
GRATZNER, HG ;
LEIF, RC ;
INGRAM, DJ ;
CASTRO, A .
EXPERIMENTAL CELL RESEARCH, 1975, 95 (01) :88-94
[7]   LOCALIZATION OF SISTER CHROMATID EXCHANGES IN HUMAN CHROMOSOMES [J].
LATT, SA .
SCIENCE, 1974, 185 (4145) :74-76
[9]   DIRECT EVIDENCE FOR ROLE OF INCORPORATED BUDR IN INDUCTION OF SISTER CHROMATID EXCHANGES [J].
MAZRIMAS, JA ;
STETKA, DG .
EXPERIMENTAL CELL RESEARCH, 1978, 117 (01) :23-30
[10]   FREQUENCY AND DISTRIBUTION OF SISTER CHROMATID EXCHANGES IN HUMAN-CHROMOSOMES [J].
MORGAN, WF ;
CROSSEN, PE .
HUMAN GENETICS, 1977, 38 (03) :271-278