Compound and metabolite distribution measured by MALDI mass spectrometric imaging in whole-body tissue sections

被引：226

作者：

Stoeckli, Markus

Staab, Dieter

Schweitzer, Alain

机构：

[1] Novartis Inst Biomed Res, CH-4002 Basel, Switzerland

[2] Novartis, Exploratory Dev, Drug Metab & Pharmacokinet, Basel, Switzerland

来源：

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY | 2007年 / 260卷 / 2-3期

关键词：

mass spectrometry; MALDI; drug imaging; metabolites;

D O I：

10.1016/j.ijms.2006.10.007

中图分类号：

O64 [物理化学（理论化学）、化学物理学]; O56 [分子物理学、原子物理学];

学科分类号：

070203 ; 070304 ; 081704 ; 1406 ;

摘要：

The determination of the compound distribution in laboratory animal tissue in early development is a standard process in pharmaceutical research. While this information is traditionally obtained by means of whole-body autoradiography using radiolabeled compounds, this technology does not distinguish between metabolites and parent compound. The technique described in this article, termed matrix-assisted laser desorption/ionization (MALDI) mass spectrometric imaging, can fill this gap by simultaneously measuring compound and multiple metabolites distributed in whole-body tissue sections, using non-labeled compounds. (C) 2006 Elsevier B.V. All rights reserved.

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收藏

页码：195 / 202

页数：8

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