Phorbol myristate acetate-dependent association of protein kinase C alpha with phospholipase D1 in intact cells

被引：64

作者：

Lee, TG

Park, JB

Lee, SD

Hong, S

Kim, JH

Kim, Y

Yi, KS

Bae, S

Hannun, YA

Obeid, LM

Suh, PG

Ryu, SH

机构：

[1] POHANG UNIV SCI & TECHNOL,DEPT LIFE SCI,POHANG 790784,SOUTH KOREA

[2] DUKE UNIV,MED CTR,DEPT MED,DURHAM,NC 27710

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM | 1997年 / 1347卷 / 2-3期

关键词：

phospholipase D1; protein kinase C alpha; antibody; immunoaffinity-purification; phorbol myristate acetate; immunoprecipitation;

D O I：

10.1016/S0005-2760(97)00083-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A phospholipase D1 (PLD1) was purified from rat brain by the use of antibody-coupled protein A Sepharose. We found that protein kinase C alpha (PKC alpha) stimulated PLD1 activity in the presence of phorbol myristate acetate (PMA). PMA-dependent association of PKC alpha with PLD1 was verified in NLH-3T3 fibroblast cells, and COS7 cells transiently expressing PLD1 as well as in vitro suggesting that the activation of PLD1 resulted from direct association of PKC alpha with PLD1. (C) 1997 Elsevier Science B.V.

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页码：199 / 204

页数：6

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