Noninvasive detection and staging of oral cancer in vivo with confocal opto-acoustic tomography

被引:25
作者
Savateeva, E [1 ]
Karabutov, A [1 ]
Motamedi, M [1 ]
Bell, B [1 ]
Johnigan, R [1 ]
Oraevsky, A [1 ]
机构
[1] Univ Texas, Med Branch, Ctr Biomed Engn, Opto Acoust Imaging & Spect Lab, Galveston, TX 77555 USA
来源
BIOMEDICAL OPTOACOUSTICS | 2000年 / 3916卷
关键词
opto-acoustic transducer; in vivo diagnostics; laser; squamous cell carcinoma; hamster model;
D O I
10.1117/12.386341
中图分类号
O42 [声学];
学科分类号
070206 ; 082403 ;
摘要
Confocal opto-acoustic transducer (COAT) was developed and applied for detection of early stages of squamous cell carcinoma in hamster model of oral cancer. COAT is a novel imaging modality with optical and acoustic lens utilized for detecting in-depth opto-acoustic profiles from a narrow cone of laser-irradiated tissue. The most important modification of COAT compared with early opto-acoustic front surface transducer (OAFST) is an improved lateral resolution of 60-mu m. The bandwidth of the confocal opto-acoustic transducer is more than 100 MHz. Therefore, in-depth axial resolution defined by the laser pulse duration and detection system equals 15-mu m. Imaging was performed at the wavelength of the Nd:YAG laser second harmonic (532 nm), which provided sufficient depth of monitoring (similar to 1.5 mm) and significant tissue contrast. Correlation of the opto-acoustic images with H&E histology sections in control animals and in animals treated with carcinogenic agent, DMBA, confirmed previous findings that early cancer lesions invisible by the naked eye may be detected with the opto-acoustic tomography. Compact design of COAT allows, in principle, application of the opto-acoustic imaging in any organ of the human digestive system.
引用
收藏
页码:55 / 66
页数:12
相关论文
共 27 条
[1]  
[Anonymous], 1993, Z1361 ANSI
[2]   Characterization of post mortem arterial tissue using time-resolved photoacoustic spectroscopy at 436, 461 and 532 nm [J].
Beard, PC ;
Mills, TN .
PHYSICS IN MEDICINE AND BIOLOGY, 1997, 42 (01) :177-198
[3]  
DACOSTA RS, 1997, J ANAL MORPHOLOGY CE, V4, P24
[4]  
Fitzmaurice M, 1999, LAB INVEST, V79, p75A
[5]   Cancer statistics, 2000 [J].
Greenlee, RT ;
Murray, T ;
Bolden, S ;
Wingo, PA .
CA-A CANCER JOURNAL FOR CLINICIANS, 2000, 50 (01) :7-33
[6]  
IZATT JA, 1999, SKIN RES TECHNOL, V5, P138
[7]   Backward mode detection of laser-induced wide-band ultrasonic transients with optoacoustic transducer [J].
Karabutov, AA ;
Savateeva, EV ;
Podymova, NB ;
Oraevsky, AA .
JOURNAL OF APPLIED PHYSICS, 2000, 87 (04) :2003-2014
[8]  
Karabutov AA, 1996, APPL PHYS B-LASERS O, V63, P545
[9]  
KARABUTOV AA, 1999, SPIE, V3601, P284
[10]   Laser optoacoustic tomography of layered tissues: Signal processing [J].
Oraevsky, AA ;
Esenaliev, RO ;
Karabutov, A .
OPTICAL TOMOGRAPHY AND SPECTROSCOPY OF TISSUE: THEORY, INSTRUMENTATION, MODEL, AND HUMAN STUDIES II, PROCEEDINGS OF, 1997, 2979 :59-70