Small heat shock protein αB-crystallin binds to p53 to sequester its translocation to mitochondria during hydrogen peroxide-induced apoptosis

被引:102
作者
Liu, Shuang
Li, Junli
Tao, Yongguang
Xiao, Xianzhong [1 ]
机构
[1] Cent S Univ, Xiangya Sch Med, Dept Pathophysiol, Chungsha 410078, Hunan, Peoples R China
[2] Cent S Univ, Xiangya Sch Med, Inst Canc Res, Chungsha 410078, Hunan, Peoples R China
关键词
alpha B-crystallin; hydrogen peroxide; p53; interaction; translocation; mitochondria; apoptosis;
D O I
10.1016/j.bbrc.2006.12.152
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Apoptosis is a highly conserved procedure of cell death and occurs under various stimuli, including oxidative stress. A small heat shock protein, alpha B-crystallin, is found to process resistance to apoptosis in some cells and tissues. But the mechanisms under this protective role are not fully understood. In the present study, we reported the early protective role of alpha B-crystallin against hydrogen peroxide-induced apoptosis in mice myogenic C2C12 cells. alpha B-Crystallin interacted with p53, a proapoptotic protein, during cell apoptosis and such protein interaction mainly occurred in the cytoplasm of the cells, suggesting that the interaction of alpha B-crystallin with p53 might prevent the translocation of p53 from cytoplasm to mitochondria. Hence, this study provides a hint that alpha B-crystallin affects on p53 mitochondrial translocation during oxidative stress-induced apoptosis. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码:109 / 114
页数:6
相关论文
共 24 条
[1]  
Alge CS, 2002, INVEST OPHTH VIS SCI, V43, P3575
[2]   'The stress of ding': the role of heat shock proteins in the regulation of apoptosis [J].
Beere, HM .
JOURNAL OF CELL SCIENCE, 2004, 117 (13) :2641-2651
[3]   Hydrogen peroxide activation of multiple mitogen-activated protein kinases in an oligodendrocyte cell line: Role of extracellular signal-regulated kinase in hydrogen peroxide-induced cell death [J].
Bhat, NR ;
Zhang, PS .
JOURNAL OF NEUROCHEMISTRY, 1999, 72 (01) :112-119
[4]   Association of the chaperone αB-crystallin with titin in heart muscle [J].
Bullard, B ;
Ferguson, C ;
Minajeva, A ;
Leake, MC ;
Gautel, M ;
Labeit, D ;
Ding, LL ;
Labeit, S ;
Horwitz, J ;
Leonard, KR ;
Linke, WA .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (09) :7917-7924
[5]   The mitochondrial death pathway and cardiac myocyte apoptosis [J].
Crow, MT ;
Mani, K ;
Nam, YJ ;
Kitsis, RN .
CIRCULATION RESEARCH, 2004, 95 (10) :957-970
[6]   Mimicking phosphorylation of the small heat-shock protein αB-crystallin recruits the F-box protein FBX4 to nuclear SC35 speckles [J].
den Engelsman, J ;
Bennink, EJ ;
Doerwald, L ;
Onnekink, C ;
Wunderink, L ;
Andley, UP ;
Kato, K ;
de Jong, WW ;
Boelens, WC .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 2004, 271 (21) :4195-4203
[7]   In vivo mitochondrial p53 transloclation triggers a rapid first wave of cell death in response to DNA damage that can precede p53 target gene activation [J].
Erster, S ;
Mihara, M ;
Kim, RH ;
Petrenko, O ;
Moll, UM .
MOLECULAR AND CELLULAR BIOLOGY, 2004, 24 (15) :6728-6741
[8]   Control of apoptosis by p53 [J].
Fridman, JS ;
Lowe, SW .
ONCOGENE, 2003, 22 (56) :9030-9040
[9]   Hydrogen peroxide as a signal controlling plant programmed cell death [J].
Gechev, TS ;
Hille, J .
JOURNAL OF CELL BIOLOGY, 2005, 168 (01) :17-20
[10]   Apoptosis - the p53 network [J].
Haupt, S ;
Berger, M ;
Goldberg, Z ;
Haupt, Y .
JOURNAL OF CELL SCIENCE, 2003, 116 (20) :4077-4085