Cloning and functional characterization of a rat urea transporter: Expression in the brain

被引：55

作者：

Couriaud, C ^{[1
]}

Ripoche, P ^{[1
]}

Rousselet, G ^{[1
]}

机构：

[1] CEA SACLAY,SERV BIOL CELLULAIRE,F-91191 GIF SUR YVETTE,FRANCE

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1996年 / 1309卷 / 03期

关键词：

urea transporter; thiourea; pCMBS; phloretin; brain; (Xenopus oocyte);

D O I：

10.1016/S0167-4781(96)00172-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA coding for a rat urea transporter is described. The 1242 bp open reading frame codes for a 414 amino acids protein with 77.0% and 60.7% identity with the human UT11 and the rat UT2, respectively. When expressed in Xenopus oocytes, the protein induces a 10-fold rise in urea permeability, inhibited by mercurial reagents and phloretin. The rUT11 mRNA is massively expressed in the brain.

引用

页码：197 / 199

页数：3

共 7 条

[1]

MARSH DJ, 1992, RENAL PHYSL, P1317

[2]

OLIVES B, 1994, J BIOL CHEM, V269, P31649

[3] Molecular characterization of a new urea transporter in the human kidney [J].

Olives, B ;

Martial, S ;

Mattei, MG ;

Matassi, G ;

Rousselet, G ;

Ripoche, P ;

Cartron, JP ;

Bailly, P .

FEBS LETTERS, 1996, 386 (2-3) :156-160

[4]

Promeneur D, 1996, J AM SOC NEPHROL, V7, P852

[5] Tandem sequence repeats in urea transporters: Identification, of an urea transporter signature sequence [J].

Rousselet, G ;

Ripoche, P ;

Bailly, P .

AMERICAN JOURNAL OF PHYSIOLOGY-RENAL FLUID AND ELECTROLYTE PHYSIOLOGY, 1996, 270 (03) :F554-F555

[6] CLONING AND REGULATION OF EXPRESSION OF THE RAT-KIDNEY UREA TRANSPORTER (RUT2) [J].

SMITH, CP ;

LEE, WS ;

MARTIAL, S ;

KNEPPER, MA ;

YOU, GF ;

SANDS, JM ;

HEDIGER, MA .

JOURNAL OF CLINICAL INVESTIGATION, 1995, 96 (03) :1556-1563

[7] CLONING AND CHARACTERIZATION OF THE VASOPRESSIN-REGULATED UREA TRANSPORTER [J].

YOU, GF ;

SMITH, CP ;

KANAI, Y ;

LEE, WS ;

STELZNER, M ;

HEDIGER, MA .

NATURE, 1993, 365 (6449) :844-847

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