A new, simple, rapid test for detection of DNase activity of microorganisms: DNase Tube test

被引：25

作者：

Gerceker, Devran ^{[1
]}

Karasartova, Djursun ^{[1
]}

Elyurek, Emel ^{[1
]}

Barkar, Saeid ^{[1
]}

Kiyan, Mehmet ^{[1
]}

Ozsan, T. Murat ^{[1
]}

Calgin, M. Kerem ^{[1
]}

Sahin, Fikret ^{[1
]}

机构：

[1] Ankara Univ, Sch Med, Dept Microbiol, TR-06100 Ankara, Turkey

来源：

JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY | 2009年 / 55卷 / 04期

关键词：

DNase test; DNase activity; Staphylococcus; DEOXYRIBONUCLEASE TEST; IDENTIFICATION; SERRATIA; NUCLEASE;

D O I：

10.2323/jgam.55.291

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

The DNase test is a simple, economical method that has traditionally been used as a supplemental test to identify pathogenic Staphylococcus. This test also aids in the differentiation of closely-related genera within the Klebsiella-Enterobacter-Serratia division of Enterobacteriaceae and several other pathogens, including screening of C. diphtheriae. Currently DNase activity of microorganisms was tested using DNase agar plate methods. These tests have some drawbacks including the necessity of the extensive time to see the results of DNase activity of bacteria. In here, we developed a new method which is simple, rapid, inexpensive and applicable to examine DNase activity of any bacteria. In this method, simply, bacteria is added to the broth medium containing DNA and followed for the DNA degradation caused by the DNase of bacteria by running in agarose gel. This method we called DNase Tube test showed DNA degradation as fast as in half an hour depending on the DNase activity of the bacteria.

引用

页码：291 / 294

页数：4

共 13 条

[1]

Methods for identification of Staphylococcus aureus isolates in cases of bovine mastitis [J].