The enterotoxin T (BcET) from Bacillus cereus can probably not contribute to food poisoning

被引：12

作者：

Choma, C ^{[1
]}

Granum, PE ^{[1
]}

机构：

[1] Norwegian Sch Vet Sci, Dept Pharmacol Microbiol & Food Hyg, N-0033 Oslo, Norway

来源：

FEMS MICROBIOLOGY LETTERS | 2002年 / 217卷 / 01期

关键词：

enterotoxin; food poisoning; Vero cell; Bacillus cereus;

D O I：

10.1016/S0378-1097(02)01046-7

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A polymerase chain reaction (PCR). fragment from strain NVH 38 (containing bceT) was cloned, sequenced and expressed in Escherichia coli. This sequence showed 50-60% identity to the original. When this bceT clone was expressed in E coli no biological activity was found in either supernatants or cell extracts. Cell extracts from the Bacillus cereus strains (NVH 38 and B-4ac) were also negative on Vero cells. Neutralisation of supernatant from B. cereus B-4ac using a monoclonal antibody (reacting with NheB and HbtD) abolished the activity. A test for cytotoxic enterotoxins was negative for both cell extracts and supernatants. Our data suggest that BcET either has an unknown type enterotoxic action or non at all. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：115 / 119

页数：5

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