A method for the isolation of schistosome eggs and miracidia free of contaminating host tissues

被引：116

作者：

Dalton, JP ^{[2
]}

Day, SR

Drew, AC

Brindley, PJ

机构：

[1] QUEENSLAND INST MED RES,BANCROFT CTR,MOL PARASITOL UNIT,ROYAL BRISBANE HOSP,HERSTON,QLD 4029,AUSTRALIA

[2] DUBLIN CITY UNIV,SCH BIOL SCI,DUBLIN 9,IRELAND

来源：

PARASITOLOGY | 1997年 / 115卷

关键词：

Schistosoma mansoni; Schistosoma japonicum; eggs; miracidia;

D O I：

10.1017/S0031182097001091

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A novel method for the isolation of schistosome eggs and miracidia from livers of mice infected with Schistosoma japonicum or S. mansoni is described. The method employed collagenase B to degrade the interstitial matrix of mouse liver tissue, after which the schistosome eggs were separated from the liver cells by 2 single-step density centrifugations through Percoll. Using this procedure sufficient quantities of miracidia were obtained to generate a cDNA library. Southern blot analysis demonstrated that miracidia isolated by this method were free from contaminating host DNA.

引用

页码：29 / 32

页数：4

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