Induction of the arginine decarboxylase ADC2 gene provides evidence for the involvement of polyamines in the wound response in arabidopsis

被引:135
作者
Perez-Amador, MA [1 ]
Leon, J
Green, PJ
Carbonell, J
机构
[1] Univ Politecn Valencia, CSIC, Inst Biol Mol & Celular Plantas, Valencia 46022, Spain
[2] Michigan State Univ, Dept Energy, Plant Res Lab, E Lansing, MI 48824 USA
[3] Michigan State Univ, Dept Biochem, E Lansing, MI 48824 USA
关键词
D O I
10.1104/pp.009951
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Polyamines are small ubiquitous molecules that have been involved in nearly all developmental processes, including the stress response. Nevertheless, no direct evidence of a role of polyamines in the wound response has been described. We have studied the expression of genes involved in polyamine biosynthesis in response to mechanical injury. An increase in the expression of the arginine decarboxylase 2 (ADC2) gene in response to mechanical wounding and methyl jasmonate (JA) treatment in Arabidopsis was detected by using DNA microarray and RNA gel-blot analysis. No induction was observed for the ADC1 gene or other genes coding for spermidine and spermine synthases, suggesting that ADC2 is the only gene of polyamine biosynthesis involved in the wounding response mediated by JA. A transient increase in the level of free putrescine followed the increase in the mRNA level for ADC2. A decrease in the level of free spermine, coincident with the increase in putrescine after wounding, was also observed. Abscisic acid effected a strong induction on ADC2 expression and had no effect on ADC1 expression. Wound-induction of ADC2 mRNA was not prevented in the JA-insensitive coil mutant. The different pattern of expression of ADC2 gene in wild-type and coil mutant might be due to the dual regulation of ADC2 by abscisic acid and JA signaling pathways. This is the first direct evidence of a function of polyamines in the wound-response, and it opens a new aspect of polyamines in plant biology.
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页码:1454 / 1463
页数:10
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