Polyribosomes are increased in spines of CA1 dendrites 2 h after the induction of LTP in mature rat hippocampal slices

被引：41

作者：

Bourne, Jennifer N. ^{[1
]}

Sorra, Karin E. ^{[1
]}

Hurlburt, Jamie ^{[1
]}

Harris, Kristen M. ^{[1
]}

机构：

[1] Med Coll Georgia, Synapses & Cognit Neurosci Ctr, Augusta, GA 30912 USA

来源：

HIPPOCAMPUS | 2007年 / 17卷 / 01期

关键词：

protein synthesis; LTP; three-dimensional reconstructions; adult; ultrastructure;

D O I：

10.1002/hipo.20238

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Enduring long-term potentiation (LTP) requires immediate protein synthesis, hence we assessed whether more polyribosomes are present in dendritic spines of mature hippocampal dendrites after the induction of LTP. Reconstructions from serial section transmission electron microscopy (sSTEM) revealed more dendritic polyribosomes 2 h posttetanus, relative to low-frequency stimulation (LFS). Polyribosomes were present in spines of all shapes with larger postsynaptic densities after 2 h, suggesting a coordinated local protein synthesis among many synapses to replenish proteins utilized during an earlier phase of LTP. (c) 2006 Wiley-Liss, Inc.

引用

页码：1 / 4

页数：4

共 16 条

[1]

Abraham WC, 2002, J NEUROSCI, V22, P9626

[2]

BOURNE JN, 2007, NEUROPHARMACOLOGY, V53, P36

[3] MORPHOLOGICAL CORRELATES OF LONG-TERM POTENTIATION IMPLY THE MODIFICATION OF EXISTING SYNAPSES, NOT SYNAPTOGENESIS, IN THE HIPPOCAMPAL DENTATE GYRUS [J].

DESMOND, NL ;

LEVY, WB .

SYNAPSE, 1990, 5 (02) :139-143

[4] Cylindrical diameters method far calibrating section thickness in serial electron microscopy [J].

Fiala, JC ;

Harris, KM .

JOURNAL OF MICROSCOPY-OXFORD, 2001, 202 (03) :468-472

[5] Reconstruct:: a free editor for serial section microscopy [J].

Fiala, JC .

JOURNAL OF MICROSCOPY-OXFORD, 2005, 218 :52-61

[6] Synaptic tagging and long-term potentiation [J].

Frey, U ;

Morris, RGM .

NATURE, 1997, 385 (6616) :533-536

[7] PRESERVATION OF NEURONAL ULTRASTRUCTURE IN HIPPOCAMPAL SLICES USING RAPID MICROWAVE-ENHANCED FIXATION [J].

JENSEN, FE ;

HARRIS, KM .

JOURNAL OF NEUROSCIENCE METHODS, 1989, 29 (03) :217-230

[8] Translational regulatory mechanisms in persistent forms of synaptic plasticity [J].

Kelleher, RJ ;

Govindarajan, A ;

Tonegawa, S .

NEURON, 2004, 44 (01) :59-73

[9]

Kirov SA, 1999, J NEUROSCI, V19, P2876

[10] Polyribosomes redistribute from dendritic shafts into spines with enlarged synapses during LTP in developing rat hippocampal slices [J].

Ostroff, LE ;

Fiala, JC ;

Allwardt, B ;

Harris, KM .

NEURON, 2002, 35 (03) :535-545

← 1 2 →