Cryo-FE-SEM & TEM immuno-techniques reveal new details for understanding white-rot decay of lignocellulose

被引:29
作者
Daniel, G
Volc, J
Niku-Paavola, ML
机构
[1] Swedish Univ Agr Sci, Wood Ultrastruct Res Ctr, SE-75007 Uppsala, Sweden
[2] Biotechnol, FIN-02044 Espoo, Finland
[3] Acad Sci Czech Republ, Inst Microbiol, Prague 14220 4, Czech Republic
关键词
white rot; Phlebia radiata; HR-Cryo FE-SEM; TEM immunocytochemistry; laccase; manganese peroxidase; diarylpropane peroxidase; extracellular slime; biodegradation; birch;
D O I
10.1016/j.crvi.2004.08.003
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
High-resolution Cryo-Field Emission Scanning Electron Microscopy (HR-Cryo-FE-SEM) and immuno-cytochemistry were used to reveal novel details on the morphological events and spatial distribution of oxidoreductive enzymes during the degradation of birch wood by the white-rot fungi Phlebia radiata and mutant strain P. radiata Cel 26. Cryo-observations of fractured fibres showed degradation across the cell wall by P radiata (wild) to progress by delamination and removal of concentric orientated aggregates from the secondary S2 cell wall. Decay by P. radiata Cel 26 progressed by removal of materials (lignin and hemicelluloses) between the aggregates (primarily cellulose) that remained even after advanced decay. With both decay patterns, extracellular slime materials were present uniting lumina hyphae with the attacked fibre wall. The extracellular slime material had two morphological forms: viz a fibrillar (often tripartite) and a 'gel-form', the former found in discrete bands progressing across the lumen onto the fibre wall. Using TEM immunocytochemistry, laccase, manganese peroxidase (MnP) and diarylpropane enzymes were localized in the periplasmic space of luminal hyphae, in association with the cell membrane, periplasmic vesicles and fungal cell wall. Extracellularly, the three enzymes were found associated with the slime and tripartite membranes and with the birch cell walls at all stages of attack through to middle lamella corner decay. Enzyme distribution was correlated with morphological changes in cell wall structure. The association of extracellular slime with these enzymes and sites of decay strongly suggests a major role for this matrix in fibre cell wall decomposition. (C) 2004 Academie des sciences. Published by Elsevier SAS. All rights reserved.
引用
收藏
页码:861 / 871
页数:11
相关论文
共 23 条
[1]  
Ander P., 1990, Microbial and enzymatic degradation of wood and wood components Ch. 2.6
[2]  
BES B, 1987, BIOTECHNOL APPL BIOC, V9, P310
[3]   STRUCTURE OF THE BETA-D-GLUCAN SECRETED BY PHANEROCHAETE-CHRYSOSPORIUM IN CONTINUOUS CULTURE [J].
BUCHALA, AJ ;
LEISOLA, M .
CARBOHYDRATE RESEARCH, 1987, 165 (01) :146-149
[4]  
DANIEL G, 1994, FEMS MICROBIOL REV, V13, P199, DOI 10.1111/j.1574-6976.1994.tb00043.x
[5]   USE OF IMMUNOGOLD CYTOCHEMISTRY TO DETECT MN(II)-DEPENDENT AND LIGNIN PEROXIDASES IN WOOD DEGRADED BY THE WHITE ROT FUNGI PHANEROCHAETE-CHRYSOSPORIUM AND LENTINULA-EDODES [J].
DANIEL, G ;
PETTERSSON, B ;
NILSSON, T ;
VOLC, J .
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 1990, 68 (04) :920-933
[6]  
Daniel G, 2003, ACS SYM SER, V845, P34
[7]   INTRACELLULAR AND EXTRACELLULAR LOCALIZATION OF LIGNIN PEROXIDASE DURING THE DEGRADATION OF SOLID WOOD AND WOOD FRAGMENTS BY PHANEROCHAETE-CHRYSOSPORIUM BY USING TRANSMISSION ELECTRON-MICROSCOPY AND IMMUNO-GOLD LABELING [J].
DANIEL, G ;
NILSSON, T ;
PETTERSSON, B .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1989, 55 (04) :871-881
[8]   PYRANOSE OXIDASE, A MAJOR SOURCE OF H2O2 DURING WOOD DEGRADATION BY PHANEROCHAETE-CHRYSOSPORIUM, TRAMETES-VERSICOLOR, AND OUDEMANSIELLA-MUCIDA [J].
DANIEL, G ;
VOLC, J ;
KUBATOVA, E .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1994, 60 (07) :2524-2532
[9]  
DANIEL G, 2004, IN PRESS P COST ACT
[10]  
Duchesne I, 2003, J PULP PAP SCI, V29, P71