Pyrosequencing for microbial typing

被引：65

作者：

Ronaghi, M

Elahi, E

机构：

[1] Stanford Univ, Stanford Genome Technol Ctr, Palo Alto, CA 94304 USA

[2] Univ Tehran, Fac Sci, Tehran, Iran

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2002年 / 782卷 / 1-2期

关键词：

reviews; pyrosequencing; genotyping; multiplexing;

D O I：

10.1016/S1570-0232(02)00693-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Pyrosequencing is a real-time DNA sequencing technique generating short reads rapidly and inexpensively. This technology has the potential advantage of accuracy, ease-of-use, high flexibility and is now emerging as a popular platform for microbial typing. Here, we review the methodology and the use of this technique for viral typing, bacteria] typing, and fungal typing. In addition, we describe how to use multiplexing for accurate and rapid typing. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

收藏

页码：67 / 72

页数：6

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