HKCNMB3 and hKCNMB4, cloning and characterization of two members of the large-conductance calcium-activated potassium channel β subunit family

被引：255

作者：

Behrens, R ^{[1
]}

Nolting, A ^{[1
]}

Reimann, F ^{[1
]}

Schwarz, M ^{[1
]}

Waldschütz, R ^{[1
]}

Pongs, O ^{[1
]}

机构：

[1] Univ Hamburg, ZMNH, Inst Neurale Signalverarbeitung, D-20246 Hamburg, Germany

来源：

FEBS LETTERS | 2000年 / 474卷 / 01期

关键词：

potassium channel; calcium-activated; accessory subunit; expression pattern;

D O I：

10.1016/S0014-5793(00)01584-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We cloned two beta subunits of large-conductance calcium-activated potassium (BK) channels, hKCNMB3 (BK beta 1) and hKCNMB4 (BK beta 4). Profiling mRNA expression showed that hKCNMB3 expression is enriched in testis and hKCNMB4 expression is very prominent in brain. We coexpressed BK channel alpha (BK alpha) and BK beta 4 subunits in vitro in CHO cells. We compared BK alpha/beta 4 mediated currents with those of smooth muscle BK alpha/beta 1 channels. BK beta 4 slowed activation kinetics more significantly, led to a steeper apparent calcium sensitivity, and shifted the voltage range of BK current activation to more negative potentials than BK beta 1. BK alpha/beta 4 channels were not blocked by 100 nM charybdotoxin or iberiotoxin, and were activated by 17 beta-estradiol. (C) 2000 Federation of European Biochemical Societies.

引用

页码：99 / 106

页数：8

共 39 条

[1] CALCIUM-ACTIVATED POTASSIUM CHANNELS EXPRESSED FROM CLONED COMPLEMENTARY DNAS
ADELMAN, JP
SHEN, KZ
KAVANAUGH, MP
WARREN, RA
WU, YN
LAGRUTTA, A
BOND, CT
NORTH, RA
[J]. NEURON, 1992, 9 (02) : 209 - 216
[2] Cloning and functional characterization of novel large conductance calcium-activated potassium channel β subunits, hKCNMB3 and hKCNMB4
Brenner, R
Jegla, TJ
Wickenden, A
Liu, Y
Aldrich, RW
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (09) : 6453 - 6461
[3] Separation of gating properties from permeation and block in mslo large conductance Ca-activated K+ channels
Cox, DH
Cui, J
Aldrich, RW
[J]. JOURNAL OF GENERAL PHYSIOLOGY, 1997, 109 (05) : 633 - 646
[4] DiChiara TJ, 1997, J NEUROSCI, V17, P4942
[5] Rat supraoptic magnocellular neurones show distinct large conductance, Ca2+-activated K+ channel subtypes in cell bodies versus nerve endings
Dopico, AM
Widmer, H
Wang, G
Lemos, JR
Treistman, SN
[J]. JOURNAL OF PHYSIOLOGY-LONDON, 1999, 519 (01): : 101 - 114
[6] Dworetzky SI, 1996, J NEUROSCI, V16, P4543
[7] CALCULATION AND CONTROL OF FREE DIVALENT-CATIONS IN SOLUTIONS USED FOR MEMBRANE-FUSION STUDIES
FOHR, KJ
WARCHOL, W
GRATZL, M
[J]. METHODS IN ENZYMOLOGY, 1993, 221 : 149 - 157
[8] GARCIACALVO M, 1994, J BIOL CHEM, V269, P676
[9] The β subunit of the high conductance calcium-activated potassium channel -: Identification of residues involved in charybdotoxin binding
Hanner, M
Vianna-Jorge, R
Kamassah, A
Schmalhofer, WA
Knaus, HG
Kaczorowski, GJ
Garcia, ML
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (26) : 16289 - 16296
[10] Human and rodent MaxiK channel β-subunit genes:: Cloning and characterization
Jiang, Z
Wallner, M
Meera, P
Toro, L
[J]. GENOMICS, 1999, 55 (01) : 57 - 67

← 1 2 3 4 →