Molecular identification and characterization of novel members of the human organic anion transporter (OATP) family

被引:507
作者
Tamai, I
Nezu, J
Uchino, H
Sai, Y
Oku, A
Shimane, M
Tsuji, A
机构
[1] Kanazawa Univ, Fac Pharmaceut Sci, Kanazawa, Ishikawa 9200934, Japan
[2] Japan Sci & Technol Corp, CREST, Kawaguchi 3320012, Japan
[3] Chugai Res Inst Mol Med Inc, Ibaraki, Osaka 3004101, Japan
关键词
D O I
10.1006/bbrc.2000.2922
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We identified three novel transporters structurally belonging to the organic anion transporting polypeptide (OATP) family in humans. Since previously known rat oatp1 to 3 do not necessarily correspond to the human OATPs in terms of either tissue distribution or function, here we designate the newly identified human OATPs as OATP-B, -D and -E, and we rename the previously known human OATP as OATP-A. OATP-C proved to be identical with the recently reported LST1/OATP-2, Expression profiles of the five OATPs and the prostaglandin transporter PGT (a member of OATP family) in human tissues showed that OATP-C is exclusively localized in liver, OATP-A and PGT are expressed in restricted ranges of tissues, and OATP-B, -D and -E show broad expression profiles. OATP-B, -C, -D and -E exhibited transport activity for [H-3]estrone-3-sulfate as a common substrate. OATP-C has a high transport activity with broad substrate specificity. (C) 2000 Academic Press.
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收藏
页码:251 / 260
页数:10
相关论文
共 32 条
[1]   Identification of a novel gene family encoding human liver-specific organic anion transporter LST-1 [J].
Abe, T ;
Kakyo, M ;
Tokui, T ;
Nakagomi, R ;
Nishio, T ;
Nakai, D ;
Nomura, H ;
Unno, M ;
Suzuki, M ;
Naitoh, T ;
Matsuno, S ;
Yawo, H .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (24) :17159-17163
[2]   Molecular characterization and tissue distribution of a new organic anion transporter subtype (oatp3) that transports thyroid hormones and taurocholate and comparison with oatp2 [J].
Abe, T ;
Kakyo, M ;
Sakagami, H ;
Tokui, T ;
Nishio, T ;
Tanemoto, M ;
Nomura, H ;
Hebert, SC ;
Matsuno, S ;
Kondo, H ;
Yawo, H .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (35) :22395-22401
[3]   EXTRACTION OF ANTIBIOTICS FROM CIRCULATION BY LIVER AND KIDNEY - EFFECT OF PROBENECID [J].
BARZA, M ;
BRUSCH, J ;
BERGERON, MG ;
KEMMOTSU, O ;
WEINSTEIN, L .
JOURNAL OF INFECTIOUS DISEASES, 1975, 131 :S86-S97
[4]   Multispecific amphipathic substrate transport by an organic anion transporter of human liver [J].
Bossuyt, X ;
Muller, M ;
Meier, PJ .
JOURNAL OF HEPATOLOGY, 1996, 25 (05) :733-738
[5]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[6]   Mechanism of prostaglandin E2 transport across the plasma membrane of HeLa cells and Xenopus oocytes expressing the prostaglandin transporter "PGT" [J].
Chan, BS ;
Satriano, JA ;
Pucci, M ;
Schuster, VL .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (12) :6689-6697
[7]  
CLAROS MG, 1994, COMPUT APPL BIOSCI, V10, P685
[8]   The sister of P-glycoprotein represents the canalicular bile salt export pump of mammalian liver [J].
Gerloff, T ;
Stieger, B ;
Hagenbuch, B ;
Madon, J ;
Landmann, L ;
Roth, J ;
Hofmann, AF ;
Meier, PJ .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (16) :10046-10050
[9]   A novel human hepatic organic anion transporting polypeptide (OATP2) - Identification of a liver-specific human organic anion transporting polypeptide and identification of rat and human hydroxymethylglutaryl-CoA reductase inhibitor transporters [J].
Hsiang, BN ;
Zhu, YJ ;
Wang, ZQ ;
Wu, YL ;
Sasseville, V ;
Yang, WP ;
Kirchgessner, TG .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (52) :37161-37168
[10]   EXPRESSION CLONING OF A RAT-LIVER NA+-INDEPENDENT ORGANIC ANION TRANSPORTER [J].
JACQUEMIN, E ;
HAGENBUCH, B ;
STIEGER, B ;
WOLKOFF, AW ;
MEIER, PJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (01) :133-137