Identification and quantification of methanogenic archaea in adult chicken ceca

被引:82
作者
Saengkerdsub, Suwat
Anderson, Robin C.
Wilkinson, Heather H.
Kim, Woo-Kyun
Nisbet, David J.
Ricke, Steven C.
机构
[1] Texas A&M Univ, Dept Poultry Sci, College Stn, TX 77843 USA
[2] USDA ARS, Southern Plain Agr Res Ctr, College Stn, TX 77845 USA
[3] Texas A&M Univ, Dept Plant Pathol & Microbiol, College Stn, TX 77845 USA
关键词
D O I
10.1128/AEM.01931-06
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
By using molecular methods for the identification and quantification of methanogenic archaea in adult chicken ceca, 16S rRNA genes of 11 different phylotypes, 10 of which were 99% similar to Methanobrevibacter woesei, were found. Methanogen populations, as assessed by cultivation, and the 16S rRNA copy number were between 6.38 and 8.23 cells/g (wet weight) and 5.50 and 7.19 log(10)/g (wet weight), respectively.
引用
收藏
页码:353 / 356
页数:4
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