The Shiga-toxin VT2-encoding bacteriophage φ297 integrates at a distinct position in the Escherichia coli genome

被引:21
作者
De Greve, H
Cao, QZ
Deboeck, F
Hernalsteens, JP
机构
[1] Free Univ Brussels VIB, Dept Immunol Parasitol & Ultrastruct, B-1640 Rhode St Genese, Belgium
[2] Free Univ Brussels, Lab Genet Virol, B-1640 Rhode St Genese, Belgium
来源
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 2002年 / 1579卷 / 2-3期
关键词
Shiga-toxin; VT2-encoding bacteriophage; attachment site; site-specific recombination; Escherichia coli O157;
D O I
10.1016/S0167-4781(02)00539-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The plaque-forming VT2-encoding lambdoid bacteriophage p297 was isolated from a Belgian clinical Escherichia coli O157:H7 isolate. PCR walking, starting from the int gene of phage phi297, demonstrated that the phi297 prophage integrated in the yecE gene of a lysogenic E. coli K12 strain. This integration site, in E. coli K12 and in the original clinical O157:H7 isolate, was confirmed by PCR using primers flanking this site. The excisionase protein of phage phi297 is identical to the excisionase of VT1-encoding phage VT1-Sakai, while the integrases, which are 82% identical, show significant sequence divergence in the central and C-terminal region. This can explain the different integration sites of both prophages. The activity of the integrase was proven by its ability to mediate the integration of a suicide plasmid, carrying the attachment site of phi297, at the appropriate position in the E. coli chromosome. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:196 / 202
页数:7
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