Megaplasmid pRme2011a of Sinorhizobium meliloti is not required for viability

被引：73

作者：

Oresnik, IJ ^{[1
]}

Liu, SL ^{[1
]}

Yost, CK ^{[1
]}

Hynes, MF ^{[1
]}

机构：

[1] Univ Calgary, Dept Biol Sci, Calgary, AB T2N 1N4, Canada

来源：

JOURNAL OF BACTERIOLOGY | 2000年 / 182卷 / 12期

关键词：

D O I：

10.1128/JB.182.12.3582-3586.2000

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We report the curing of the 1,360-kb megaplasmid pRme2011a from Sinorhizobium meliloti strain Rm2011. With a positive selection strategy that utilized Tn5B12-S containing the sacB gene, we were able to cure this replicon by successive rounds of selecting for deletion formation in vivo. Subsequent Southern blot, Eckhardt gel, and pulsed-held gel electrophoresis analyses were consistent with the hypothesis that the resultant strain was indeed missing pRme2011a. The cured derivative grew as well as the wild-type strain in both complex and defined media but was unable to use a number of substrates as a sole source of carbon on defined media.

引用

页码：3582 / 3586

页数：5

共 40 条

[1] UTILIZATION OF CARBON SUBSTRATES, ELECTROPHORETIC ENZYME PATTERNS, AND SYMBIOTIC PERFORMANCE OF PLASMID-CURED CLOVER RHIZOBIA [J].

BALDANI, JI ;

WEAVER, RW ;

HYNES, MF ;

EARDLY, BD .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (07) :2308-2314

[2] RHIZOBIUM-MELILOTI CARRIES 2 MEGAPLASMIDS [J].

BANFALVI, Z ;

KONDOROSI, E ;

KONDOROSI, A .

PLASMID, 1985, 13 (02) :129-138

[3]

BANFALVI Z, 1981, MOL GEN GENET, V184, P318

[4] A phosphate transport system is required for symbiotic nitrogen fixation by Rhizobium meliloti [J].

Bardin, S ;

Dan, S ;

Osteras, M ;

Finan, TM .

JOURNAL OF BACTERIOLOGY, 1996, 178 (15) :4540-4547

[5] Identification and characterization of a gene on Rhizobium meliloti pSyma, syrB, that negatively affects syrM expression [J].

Barnett, MJ ;

Long, SR .

MOLECULAR PLANT-MICROBE INTERACTIONS, 1997, 10 (05) :550-559

[6] LOCALIZATION OF A SYMBIOTIC FIX REGION ON RHIZOBIUM-MELILOTI PSYM MEGAPLASMID MORE THAN 200 KILOBASES FROM THE NOD-NIF REGION [J].

BATUT, J ;

TERZAGHI, B ;

GHERARDI, M ;

HUGUET, M ;

TERZAGHI, E ;

GARNERONE, AM ;

BOISTARD, P ;

HUGUET, T .

MOLECULAR AND GENERAL GENETICS, 1985, 199 (02) :232-239

[7]

BOIVIN C, 1990, PLANT CELL, V2, P1157, DOI 10.1105/tpc.2.12.1157

[8] VISUALIZATION AND EXACT MOLECULAR-WEIGHT DETERMINATION OF A RHIZOBIUM-MELILOTI MEGAPLASMID [J].

BURKARDT, B ;

BURKARDT, HJ .

JOURNAL OF MOLECULAR BIOLOGY, 1984, 175 (02) :213-218

[9] PHYSICAL CHARACTERIZATION OF RHIZOBIUM-MELILOTI MEGAPLASMIDS [J].

BURKARDT, B ;

SCHILLIK, D ;

PUHLER, A .

PLASMID, 1987, 17 (01) :13-25

[10] A high-density physical map of Sinorhizobium meliloti 1021 chromosome derived from bacterial artificial chromosome library [J].

Capela, D ;

Barloy-Hubler, F ;

Gatius, MT ;

Gouzy, J ;

Galibert, F .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (16) :9357-9362

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