Comparative genomic and phylogeographic analysis of Mycobacterium leprae

被引：288

作者：

Monot, Marc ^{[2
]}

Honore, Nadine ^{[2
]}

Garnier, Thierry ^{[2
]}

Zidane, Nora ^{[2
]}

Sherafi, Diana ^{[2
]}

Paniz-Mondolfi, Alberto ^{[3
]}

Matsuoka, Masanori ^{[4
]}

Taylor, G. Michael ^{[5
]}

Donoghue, Helen D. ^{[5
]}

Bouwman, Abi ^{[6
]}

Mays, Simon ^{[7
]}

Watson, Claire ^{[8
]}

Lockwood, Diana ^{[8
]}

Khamispour, Ali ^{[9
]}

Dowlati, Yahya ^{[9
]}

Shen Jianping ^{[10
]}

Rea, Thomas H. ^{[11
]}

Vera-Cabrera, Lucio ^{[12
]}

Stefani, Mariane M. ^{[13
]}

Banu, Sayera ^{[14
]}

Macdonald, Murdo ^{[15
]}

Sapkota, Bishwa Raj ^{[15
]}

Spencer, John S. ^{[16
]}

Thomas, Jerome ^{[17
]}

Harshman, Keith ^{[17
]}

Singh, Pushpendra ^{[1
]}

Busso, Philippe ^{[1
]}

Gattiker, Alexandre ^{[18
]}

Rougemont, Jacques ^{[18
]}

Brennan, Patrick J. ^{[16
]}

Cole, Stewart T. ^{[1
]}

机构：

[1] Ecole Polytech Fed Lausanne, Global Hlth Inst, Lausanne, Switzerland

[2] Inst Pasteur, Paris, France

[3] Inst Biomed, Caracas, Venezuela

[4] Natl Inst Infect Dis, Leprosy Res Ctr, Tokyo, Japan

[5] UCL, Windeyer Inst, Ctr Infect Dis & Int Hlth, London, England

[6] Univ Manchester, Fac Life Sci, Manchester Interdisciplinary Bioctr, Manchester, Lancs, England

[7] English Heritage Ctr Archaeol, Ancient Monuments Lab, Portsmouth, Hants, England

[8] London Sch Hyg & Trop Med, London WC1, England

[9] Univ Tehran Med Sci, Ctr Res & Training Skin Dis & Leprosy, Tehran, Iran

[10] China Ctr Dis Control & Prevent, Natl Ctr Leprosy Control, Nanjing, Peoples R China

[11] Univ So Calif, Keck Sch Med, Dept Dermatol, Los Angeles, CA 90033 USA

[12] Hosp Univ Jose E Gonzalez, Serv Dermatol, Monterrey, Nuevo Leon, Mexico

[13] Univ Fed Goias, Setor Univ, Trop Pathol & Publ Hlth Inst, Goiania, Go, Brazil

[14] Int Ctr Diarrhoeal Dis Res, Dhaka 1000, Bangladesh

[15] Anandaban Hosp, Kathmandu, Nepal

[16] Colorado State Univ, Dept Microbiol Immunol & Pathol, Ft Collins, CO 80523 USA

[17] Univ Lausanne, Ctr Integrat Genom, DNA Array Facil, Lausanne, Switzerland

[18] Ecole Polytech Fed Lausanne, Bioinformat & Biostat Core Facil, Lausanne, Switzerland

来源：

NATURE GENETICS | 2009年 / 41卷 / 12期

基金：

美国国家卫生研究院;

关键词：

NUMBER TANDEM REPEATS; LEPROSY PATIENTS; CAUSATIVE AGENT; SEQUENCE; EVOLUTION; TUBERCULOSIS; DIVERSITY; ALIGNMENT; ULCERANS; INSIGHTS;

D O I：

10.1038/ng.477

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Reductive evolution and massive pseudogene formation have shaped the 3.31-Mb genome of Mycobacterium leprae, an unculturable obligate pathogen that causes leprosy in humans. The complete genome sequence of M. leprae strain Br4923 from Brazil was obtained by conventional methods (6 x coverage), and Illumina resequencing technology was used to obtain the sequences of strains Thai53 (38 x coverage) and NHDP63 (46 x coverage) from Thailand and the United States, respectively. Whole-genome comparisons with the previously sequenced TN strain from India revealed that the four strains share 99.995% sequence identity and differ only in 215 polymorphic sites, mainly SNPs, and by 5 pseudogenes. Sixteen interrelated SNP subtypes were defined by genotyping both extant and extinct strains of M. leprae from around the world. The 16 SNP subtypes showed a strong geographical association that reflects the migration patterns of early humans and trade routes, with the Silk Road linking Europe to China having contributed to the spread of leprosy.

引用

页码：1282 / U39

页数：10

共 66 条

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