Insect Infection Model for Campylobacter jejuni Reveals That O-methyl Phosphoramidate Has Insecticidal Activity

被引:66
作者
Champion, Olivia L. [1 ]
Karlyshev, Andrey V. [2 ]
Senior, Nicola J. [1 ]
Woodward, Martin [4 ]
La Ragione, Roberto [4 ]
Howard, Sarah L. [3 ]
Wren, Brendan W. [3 ]
Titball, Richard W. [1 ]
机构
[1] Univ Exeter, Sch Biosci, Exeter EX4 4QD, Devon, England
[2] Kingston Univ, Sch Life Sci, London, England
[3] London Sch Hyg & Trop Med, Dept Infect & Trop Dis, London WC1, England
[4] Vet Labs Agcy, Dept Food & Environm Safety, Addlestone, Surrey, England
关键词
GALLERIA-MELLONELLA; EPITHELIAL-CELLS; COLONIZATION; VIRULENCE; SYSTEM; MUTANTS; TOXIN; HOST; MICE; POLYSACCHARIDE;
D O I
10.1086/650494
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Galleria mellonella (wax moth) larvae have elsewhere been shown to be susceptible to pathogens such as Francisella tularensis, Burkholderia mallei, and Pseudomonas aeruginosa. We report that the larvae are rapidly killed by Campylobacter jejuni at 37 degrees C. Three strains of C. jejuni tested, 11168H (human diarrheal isolate), G1 (human Guillain-Barre syndrome isolate), and 81-176 (human diarrheal isolate), were equally effective at killing G. mellonella larvae. A panel of defined mutants of C. jejuni 11168H, in known or putative virulence genes, showed different degrees of attenuation in G. mellonella larvae. A mutant lacking the O-methyl phosphoramidate (MeOPN) capsule side group was attenuated, clearly demonstrating that MeOPN has a role in virulence. This new model of C. jejuni infection should facilitate the identification of novel virulence genes.
引用
收藏
页码:776 / 782
页数:7
相关论文
共 44 条
[1]  
Allos BM, 2001, CLIN INFECT DIS, V32, P1201, DOI 10.1086/319760
[2]   Galleria mellonella as a model host to study infection by the Francisella tularensis live vaccine strain [J].
Aperis, George ;
Fuchs, Beth Burgwyn ;
Anderson, Christine A. ;
Warner, John E. ;
Calderwood, Stephen B. ;
Mylonakis, Eleftherios .
MICROBES AND INFECTION, 2007, 9 (06) :729-734
[3]   NEWBORN PIGLET MODEL FOR CAMPYLOBACTERIOSIS [J].
BABAKHANI, FK ;
BRADLEY, GA ;
JOENS, LA .
INFECTION AND IMMUNITY, 1993, 61 (08) :3466-3475
[4]   A phase-variable capsule is involved in virulence of Campylobacter jejuni 81-176 [J].
Bacon, DJ ;
Szymanski, CM ;
Burr, DH ;
Silver, RP ;
Alm, RA ;
Guerry, P .
MOLECULAR MICROBIOLOGY, 2001, 40 (03) :769-777
[5]   Superoxide production in Galleria mellonella hemocytes:: Identification of proteins homologous to the NADPH oxidase complex of human neutrophils [J].
Bergin, D ;
Reeves, EP ;
Renwick, J ;
Wientjes, FB ;
Kavanagh, K .
INFECTION AND IMMUNITY, 2005, 73 (07) :4161-4170
[6]  
Brennan M, 2002, FEMS IMMUNOL MED MIC, V34, P153, DOI 10.1111/j.1574-695X.2002.tb00617.x
[7]   A murine intraperitoneal infection model reveals that host resistance to Campylobacter jejuni is Nramp 1 dependent [J].
Champion, Olivia L. ;
Valdez, Yanet ;
Thorson, Lisa ;
Guttman, Julian A. ;
Menendez, Alfredo ;
Gaynor, Erin C. ;
Finlay, B. Brett .
MICROBES AND INFECTION, 2008, 10 (08) :922-927
[8]   Campylobacter jejuni colonization of mice with limited enteric flora [J].
Chang, Christopher ;
Miller, Jeff F. .
INFECTION AND IMMUNITY, 2006, 74 (09) :5261-5271
[9]  
Darby Creg, 2005, WormBook, P1
[10]  
Davis L., 2008, Curr. Protoc. Microbiol. Chapter, V8, p8A.2.1