Diagnostic tools based on minor groove binder probe technology for rapid identification of vaccinal and field strains of canine parvovirus type 2b

被引：49

作者：

Decaro, Nicola ^{[1
]}

Martella, Vito ^{[1
]}

Elia, Gabriella ^{[1
]}

Desario, Costantina ^{[1
]}

Campolo, Marco ^{[1
]}

Buonavoglia, Domenico ^{[1
]}

Bellacicco, Anna Lucia ^{[1
]}

Tempesta, Maria ^{[1
]}

Buonavoglia, Canio ^{[1
]}

机构：

[1] Univ Bari, Fac Med Vet, Dept Anim Hlth & Well Being, I-70010 Bari, Italy

来源：

JOURNAL OF VIROLOGICAL METHODS | 2006年 / 138卷 / 1-2期

关键词：

dog; parvovirus; type 2b vaccines; type 2b field strains; MGB probe assays;

D O I：

10.1016/j.jviromet.2006.07.011

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

TaqMan-based diagnostic tests have been developed for the identification of canine parvovirus type 2 (CPV-2) strains in the faeces of dogs with diarrhoea, including a minor groove binder (MGB) probe assay for identification of type 2-based vaccines and field strains (types 2a, 2b and 2c). Since type 2b vaccines have been licensed recently in Europe, two novel MGB assays were developed for discrimination between type 2b vaccines and field strains of CPV. Such assays have been found to be highly sensitive, specific and reproducible, allowing for simultaneous detection of type 2b vaccinal and field strains present in the same specimens. These new assays will help resolution of the diagnostic problems related to the detection of a type 2b strain in the faeces of dogs shortly after the administration of a type 2b vaccine. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：10 / 16

页数：7

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