Analysis of the Plasmodium falciparum proteome by high-accuracy mass spectrometry

被引:515
作者
Lasonder, E
Ishihama, Y
Andersen, JS
Vermunt, AMW
Pain, A
Sauerwein, RW
Eling, WMC
Hall, N
Waters, AP
Stunnenberg, HG
Mann, M
机构
[1] Univ Nijmegen, Dept Mol Biol, NCMLS, NL-6525 GA Nijmegen, Netherlands
[2] Univ So Denmark, Ctr Expt Bioinformat, Dept Biochem & Mol Biol, DK-5230 Odense M, Denmark
[3] Wellcome Trust Sanger Inst, Cambridge CB10 1SA, England
[4] Univ Med Ctr, Dept Med Microbiol, NCMLS, NL-6500 HB Nijmegen, Netherlands
[5] Leiden Univ, Leiden Malaria Res Grp, Med Ctr, Dept Parasitol, Ctr Infect Dis, NL-2333 ZA Leiden, Netherlands
关键词
D O I
10.1038/nature01111
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The annotated genomes of organisms define a 'blueprint' of their possible gene products. Post-genome analyses attempt to confirm and modify the annotation and impose a sense of the spatial, temporal and developmental usage of genetic information by the organism. Here we describe a large-scale, high-accuracy (average deviation less than 0.02 Da at 1,000 Da) mass spectrometric proteome analysis(1-3) of selected stages of the human malaria parasite Plasmodium falciparum. The analysis revealed 1,289 proteins of which 714 proteins were identified in asexual blood stages, 931 in gametocytes and 645 in gametes. The last two groups provide insights into the biology of the sexual stages of the parasite, and include conserved, stage-specific, secreted and membrane-associated proteins. A subset of these proteins contain domains that indicate a role in cell-cell interactions, and therefore can be evaluated as potential components of a malaria vaccine formulation. We also report a set of peptides with significant matches in the parasite genome but not in the protein set predicted by computational methods.
引用
收藏
页码:537 / 542
页数:6
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