Enhanced DNA repair of cyclobutane pyrimidine dimers changes the biological response to UV-B radiation

被引：19

作者：

Yarosh, DB ^{[1
]}

机构：

[1] Appl Genet Inc Dermat, Freeport, NY 11520 USA

来源：

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS | 2002年 / 509卷 / 1-2期

关键词：

UV; excision repair; T4 endonuclease V; liposomes; cyclobutane pyrimidine dimers; skin cancer;

D O I：

10.1016/S0027-5107(02)00215-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The goal of DNA repair enzyme therapy is the same as that for gene therapy: to rescue a defective proteome/genome by introducing a substitute protein/DNA. The danger of inadequate DNA repair is highlighted in the genetic disease xeroderma pigmentosum. These patients are hypersensitive to sunlight and develop multiple cutaneous neoplasms very early in life. The bacterial DNA repair enzyme T4 endonuclease V was shown over 25 years ago to be capable of reversing the defective repair in xeroderma pigmentosum cells. This enzyme, packaged in an engineered delivery vehicle, has been shown to traverse the stratum corneum, reach the nuclei of living cells of the skin, and enhance the repair of UV-induced cyclobutane pyrimidine dimers (CPD). In such a system, changes in DNA repair, mutagenesis, and cell signaling can be studied without manipulation of the genome., (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：221 / 226

页数：6

共 32 条

[1] PARTIAL COMPLEMENTATION OF THE UV SENSITIVITY OF ESCHERICHIA-COLI AND YEAST EXCISION REPAIR MUTANTS BY THE CLONED DENV GENE OF BACTERIOPHAGE-T4 [J].