Deciphering soluble and membrane protein function using yeast systems

被引：8

作者：

Bao, Leyuan ^{[1
,2
]}

Redondo, Clara ^{[3
]}

Findlay, John B. C. ^{[3
]}

Walker, John H. ^{[1
,2
]}

Ponnambalam, Sreenivasan ^{[1
,2
]}

机构：

[1] Univ Leeds, Fac Biol Sci, Endothelial Cell Biol Unit, Leeds LS2 9JT, W Yorkshire, England

[2] Univ Leeds, Fac Biol Sci, Inst Mol & Cellular Biol, Leeds LS2 9JT, W Yorkshire, England

[3] Univ Leeds, Fac Biol Sci, Inst Membrane & Syst Biol, Leeds Inst Genet Hlth & Therapeut, Leeds LS2 9JT, W Yorkshire, England

来源：

MOLECULAR MEMBRANE BIOLOGY | 2009年 / 26卷 / 03期

基金：

英国惠康基金;

关键词：

Protein-protein interaction; yeast two-hybrid; yeast three-hybrid; membrane protein; drug discovery; SPLIT-UBIQUITIN SYSTEM; INTERACTIONS IN-VIVO; GENOME-WIDE ANALYSIS; GENETIC SYSTEM; 2-HYBRID SYSTEM; SCREENING SYSTEM; IDENTIFICATION; INHIBITORS; IDENTIFY; PATHWAY;

D O I：

10.1080/09687680802637652

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Membrane protein-protein interactions are important for regulation, targeting, and activity of proteins in membranes but are difficult to detect and analyse. This review covers current approaches to studying membrane protein interactions. In addition to standard biochemical and genetic techniques, the classic yeast nuclear two-hybrid system has been highly successful in identification and characterization of soluble protein-protein interactions. However, classic yeast two-hybrid assays do not work for membrane proteins because such yeast-based interactions must occur in the nucleus. Here, we highlight recent advances in yeast systems for the detection and characterization of eukaryote membrane protein-protein interactions. We discuss these implications for drug screening and discovery.

引用

页码：127 / 135

页数：9

共 59 条

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