A recessive mutant cell line with a constitutive IκB kinase activity

被引：7

作者：

Chinanonwait, N ^{[1
]}

Miura, H ^{[1
]}

Yamamoto, N ^{[1
]}

Yamaoka, S ^{[1
]}

机构：

[1] Tokyo Med & Dent Univ, Grad Sch, Dept Mol Virol, Bunkyo Ku, Tokyo 1138519, Japan

来源：

FEBS LETTERS | 2002年 / 531卷 / 03期

关键词：

recessive mutation; NF-kappa B; IKK activation;

D O I：

10.1016/S0014-5793(02)03618-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To search for negative regulatory components of the NF-kappaB activation pathways, we mutagenized Rat-1 fibroblasts and established a stable mutant cell line with a constitutive NF-kappaB activity. This mutant cell line, designated as TK26, showed permanently elevated I kappa B kinase (IKK) activity and a genetically recessive phenotype revealed by somatic cell hybridization between TK26 and Rat-1. Our results suggested that lack of a negative regulation of IKK could lead to permanent NF-kappaB activation. The TK26 cell line will be useful to genetically identify a component necessary for keeping the IKK complex under an inactive form in resting cells. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：553 / 560

页数：8

共 32 条

[1] NF-kappa B: Ten years after
Baeuerle, PA
Baltimore, D
[J]. CELL, 1996, 87 (01) : 13 - 20
[2] The NF-kappa B and I kappa B proteins: New discoveries and insights
Baldwin, AS
[J]. ANNUAL REVIEW OF IMMUNOLOGY, 1996, 14 : 649 - 683
[3] Persistent activation of NF-κB by the tax transforming protein involves chronic phosphorylation of IκB kinase subunits IKKβ and IKKγ
Carter, RS
Geyer, BC
Xie, MH
Acevedo-Suárez, CA
Ballard, DW
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (27) : 24445 - 24448
[4] NEMO/IKKγ:: Linking NF-κB to human disease
Courtois, G
Smahi, A
Israël, A
[J]. TRENDS IN MOLECULAR MEDICINE, 2001, 7 (10) : 427 - 430
[5] DiDonato J, 1996, MOL CELL BIOL, V16, P1295
[6] A cytokine-responsive I kappa B kinase that activates the transcription factor NF-kappa B
DiDonato, JA
Hayakawa, M
Rothwarf, DM
Zandi, E
Karin, M
[J]. NATURE, 1997, 388 (6642) : 548 - 554
[7] X-linked anhidrotic ectodermal dysplasia with immunodeficiency is caused by impaired NF-κB signaling
Döffinger, R
Smahi, A
Bessia, C
Geissmann, F
Feinberg, J
Durandy, A
Bodemer, C
Kenwrick, S
Dupuis-Girod, S
Blanche, S
Wood, P
Rabia, SH
Headon, DJ
Overbeek, PA
Le Deist, F
Holland, SM
Belani, K
Kumararatne, DS
Fischer, A
Shapiro, R
Conley, ME
Reimund, E
Kalhoff, H
Abinun, M
Munnich, A
Israël, A
Courtois, G
Casanova, JL
[J]. NATURE GENETICS, 2001, 27 (03) : 277 - 285
[8] Diverse agents act at multiple levels to inhibit the Rel/NF-κB signal transduction pathway
Epinat, JC
Gilmore, TD
[J]. ONCOGENE, 1999, 18 (49) : 6896 - 6909
[9] Introduction: The Rel/NF-kappa B signal transduction pathway
Gilmore, TD
[J]. SEMINARS IN CANCER BIOLOGY, 1997, 8 (02) : 61 - 62
[10] INTRAMOLECULAR MASKING OF THE NUCLEAR LOCATION SIGNAL AND DIMERIZATION DOMAIN IN THE PRECURSOR FOR THE P50 NF-KAPPA-B SUBUNIT
HENKEL, T
ZABEL, U
VANZEE, K
MULLER, JM
FANNING, E
BAEUERLE, PA
[J]. CELL, 1992, 68 (06) : 1121 - 1133

← 1 2 3 4 →