Nucleosome mobility and the maintenance of nucleosome positioning

被引：83

作者：

Pazin, MJ

Bhargava, P

Geiduschek, EP

Kadonaga, JT

机构：

[1] UNIV CALIF SAN DIEGO, DEPT BIOL, LA JOLLA, CA 92093 USA

[2] UNIV CALIF SAN DIEGO, CTR MOL GENET, LA JOLLA, CA 92093 USA

[3] CTR CELLULAR & MOL BIOL, HYDERABAD 500007, ANDHRA PRADESH, INDIA

来源：

SCIENCE | 1997年 / 276卷 / 5313期

关键词：

D O I：

10.1126/science.276.5313.809

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

To study nucleosome mobility and positioning, the R3 lac repressor was used with an adenosine triphosphate (ATP)-dependent chromatin assembly system to establish the positioning of five nucleosomes, with one nucleosome located between two R3 lac operators, When R3 protein was dissociated from DNA with isopropyl beta-D-thiogalactopyranoside, the R3-induced nucleosome positions remained unchanged for at least 60 minutes in the absence of ATP but rearranged within 15 minutes in the presence of ATP. These results suggest that nucleosomes are dynamic and mobile rather than static and that a DNA binding factor is continuously required for the maintenance of nucleosome positioning.

引用

页码：809 / 812

页数：4

共 43 条

[1] GENETIC-ANALYSIS OF THE LEUCINE HEPTAD REPEATS OF LAC REPRESSOR - EVIDENCE FOR A 4-HELICAL BUNDLE [J].