Transposon-tagged mutagenesis in the rat

被引：70

作者：

Kitada, Kazuhiro

Ishishita, Satoshi

Tosaka, Keiko

Takahashi, Ri-ichi

Ueda, Masatsugu

Keng, Vincent W.

Horie, Kyoji

Takeda, Junji

机构：

[1] Hokkaido Univ, Grad Sch Environm Earth Sci, Kita Ku, Sapporo, Hokkaido 0600810, Japan

[2] Hokkaido Univ, Creat Res Initiat Sousei, Div Human Genet, Mammalian Genet Lab, Sapporo, Hokkaido 0600810, Japan

[3] Hokkaido Univ, Grad Sch Environm Earth Sci, Div Biosci, Sapporo, Hokkaido 0600810, Japan

[4] YS Inst Inc, Utsunomiya, Tochigi 3210973, Japan

[5] Osaka Univ, Grad Sch Med, Ctr Adv Sci & Innovat, Suita, Osaka 5650871, Japan

[6] Osaka Univ, Grad Sch Med, Dept Social & Environm Med, Suita, Osaka 5650871, Japan

来源：

NATURE METHODS | 2007年 / 4卷 / 02期

关键词：

D O I：

10.1038/nmeth1002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Although the laboratory rat (Rattus norvegicus) is an indispensable experimental animal for biomedical research and drug development, the lack of embryonic stem cell lines hampers gene-knockout studies. Here we report the successful generation of insertional mutant rats using the Sleeping Beauty (SB) transposon system. This would benefit a variety of biomedical research fields for which the rat model is better suited than the mouse model.

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收藏

页码：131 / 133

页数：3

相关论文

共 14 条

[1] Insertional mutagenesis in mice: New perspectives and tools [J].

Carlson, CM ;

Largaespada, DA .

NATURE REVIEWS GENETICS, 2005, 6 (07) :568-580

[2] Cancer gene discovery in solid tumours using transposon-based somatic mutagenesis in the mouse [J].

Collier, LS ;

Carlson, CM ;

Ravimohan, S ;

Dupuy, AJ ;

Largaespada, DA .

NATURE, 2005, 436 (7048) :272-276

[3] Mammalian mutagenesis using a highly mobile somatic Sleeping Beauty transposon system [J].

Dupuy, AJ ;

Akagi, K ;

Largaespada, DA ;

Copeland, NG ;

Jenkins, NA .

NATURE, 2005, 436 (7048) :221-226

[4] Transposition and gene disruption in the male germline of the mouse [J].

Dupuy, AJ ;

Fritz, S ;

Largaespada, DA .

GENESIS, 2001, 30 (02) :82-88

[5] Regulated transposition of a fish transposon in the mouse germ line [J].

Fischer, SEJ ;

Wienholds, E ;

Plasterk, RHA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (12) :6759-6764

[6] Genome sequence of the Brown Norway rat yields insights into mammalian evolution [J].

Gibbs, RA ;

Weinstock, GM ;

Metzker, ML ;

Muzny, DM ;

Sodergren, EJ ;

Scherer, S ;

Scott, G ;

Steffen, D ;

Worley, KC ;

Burch, PE ;

Okwuonu, G ;

Hines, S ;

Lewis, L ;

DeRamo, C ;

Delgado, O ;

Dugan-Rocha, S ;

Miner, G ;

Morgan, M ;

Hawes, A ;

Gill, R ;

Holt, RA ;

Adams, MD ;

Amanatides, PG ;

Baden-Tillson, H ;

Barnstead, M ;

Chin, S ;

Evans, CA ;

Ferriera, S ;

Fosler, C ;

Glodek, A ;

Gu, ZP ;

Jennings, D ;

Kraft, CL ;

Nguyen, T ;

Pfannkoch, CM ;

Sitter, C ;

Sutton, GG ;

Venter, JC ;

Woodage, T ;

Smith, D ;

Lee, HM ;

Gustafson, E ;

Cahill, P ;

Kana, A ;

Doucette-Stamm, L ;

Weinstock, K ;

Fechtel, K ;

Weiss, RB ;

Dunn, DM ;

Green, ED .

NATURE, 2004, 428 (6982) :493-521

[7] SLIT2-mediated ROBO2 signaling restricts kidney induction to a single site [J].

Grieshammer, U ;

Ma, L ;

Plump, AS ;

Wang, F ;

Tessier-Lavigne, M ;

Martin, GR .

DEVELOPMENTAL CELL, 2004, 6 (05) :709-717

[8] Characterization of Sleeping Beauty transposition and its application to genetic screening in mice [J].

Horie, K ;

Yusa, K ;

Yae, K ;

Odajima, J ;

Fischer, SEJ ;

Keng, VW ;

Hayakawa, T ;

Mizuno, S ;

Kondoh, G ;

Ijiri, T ;

Matsuda, Y ;

Plasterk, RHA ;

Takeda, J .

MOLECULAR AND CELLULAR BIOLOGY, 2003, 23 (24) :9189-9207

[9] Efficient chromosomal transposition of a Tc1/mariner-like transposon Sleeping Beauty in mice [J].

Horie, K ;

Kuroiwa, A ;

Ikawa, M ;

Okabe, M ;

Kondoh, G ;

Matsuda, Y ;

Takeda, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (16) :9191-9196

[10] Molecular reconstruction of Sleeping beauty, a Tc1-like transposon from fish, and its transposition in human cells [J].

Ivics, Z ;

Hackett, PB ;

Plasterk, RH ;

Izsvak, Z .

CELL, 1997, 91 (04) :501-510