A rapid and simple method for extracting human immunodeficiency virus type 1 RNA from plasma: Enhanced sensitivity

被引：69

作者：

Mulder, J

Resnick, R

Saget, B

Scheibel, S

Herman, S

Payne, H

Harrigan, R

Kwok, S

机构：

[1] ROCHE MOL SYST,SOMERVILLE,NJ

[2] WELLCOME RES LABS,BECKENHAM BR3 3BS,KENT,ENGLAND

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1997年 / 35卷 / 05期

关键词：

D O I：

10.1128/JCM.35.5.1278-1280.1997

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We present here a rapid and simple technique for processing human immunodeficiency virus (HIV)-infected plasma by high-speed centrifugation. HIV type 1 virions are pelleted from up to 1 ml of plasma, gently lysed with a nonionic detergent, and directly amplified. The procedure has few manipulations and requires approximately 1.5 h for the processing of 24 samples. Viral recovery ranges from 80 to 90%, with an analytical sensitivity approaching 20 copies/ml.

引用

页码：1278 / 1280

页数：3

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BUSSO M, 1994, J VIROL METHODS, V470, P129

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