Imaging of Lipids in Spinal Cord Using Intermediate Pressure Matrix-Assisted Laser Desorption-Linear Ion Trap/Orbitrap MS

被引：87

作者：

Landgraf, Rachelle R. ^{[1
]}

Conaway, Maria C. Prieto ^{[2
]}

Garrett, Timothy J. ^{[1
]}

Stacpoole, Peter W. ^{[1
]}

Yost, Richard A. ^{[1
]}

机构：

[1] Univ Florida, Gainesville, FL 32611 USA

[2] Thermo Fisher Sci, San Jose, CA 95134 USA

来源：

ANALYTICAL CHEMISTRY | 2009年 / 81卷 / 20期

关键词：

MASS-SPECTROMETRY; TISSUE-SECTIONS; SMALL MOLECULES; MALDI; METABOLITES; IONIZATION; BRAIN; PEPTIDES; DRUGS; COMBINATION;

D O I：

10.1021/ac901387u

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A hybrid linear ion trap/Orbitrap mass spectrometer was used to perform tandem mass spectrometry (MS/MS) experiments and high-resolution mass analysis of lipids desorbed from nerve tissue. A dramatic improvement in mass spectral resolution and a decrease in background are observed in the spectra collected from the Orbitrap mass analyzer, which allows generation of more accurate mass spectrometric images of the distribution of lipids within nerve tissue. Employment of both mass analyzers provides a rapid and reliable means of compound identification based on MS/MS fragmentation and high-resolution mass spectrometry (HRMS) accurate mass.

引用

收藏

页码：8488 / 8495

页数：8

相关论文

共 36 条

[1]

AGRANOFF BW, 1998, BASIC NEUROCHEMISTRY, pCH3

[2] Lipid imaging in the zebra finch brain with secondary ion mass spectrometry [J].

Amaya, Kensey R. ;

Monroe, Eric B. ;

Sweedler, Jonathan V. ;

Clayton, David F. .

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 2007, 260 (2-3) :121-127

[3] Automated MALDI matrix deposition method with inkjet printing for imaging mass spectrometry [J].

Baluya, Dodge L. ;

Garrett, Timothy J. ;

Yost, Richard A. .

ANALYTICAL CHEMISTRY, 2007, 79 (17) :6862-6867

[4] Molecular imaging of lipids in cells and tissues [J].

Borner, Katrin ;

Malmberg, Per ;

Mansson, Jan-Eric ;

Nygren, Hakan .

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 2007, 260 (2-3) :128-136

[5] Direct profiling of myelinated and demyelinated regions in mouse brain by imaging mass spectrometry [J].

Ceuppens, Ruben ;

Dumont, Debora ;

Van Brussel, Leen ;

Van de Plas, Babs ;

Daniels, Ruth ;

Noben, Jean-Paul ;

Verhaert, Peter ;

Van der Gucht, Estel ;

Robben, Johan ;

Clerens, Stefan ;

Arckens, Lutgarde .

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 2007, 260 (2-3) :185-194

[6] Colloidal graphite-assisted laser desorption/ionization mass spectrometry and MSn of small molecules.: 1.: Imaging of cerebrosides directly from rat brain tissue [J].

Cha, Sangwon ;

Yeung, Edward S. .

ANALYTICAL CHEMISTRY, 2007, 79 (06) :2373-2385

[7] A comparison of negative and positive ion time-of-flight post-source decay mass spectrometry for peptides containing basic residues [J].

Clipston, NL ;

Jai-nhuknan, J ;

Cassady, CJ .

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 2003, 222 (1-3) :363-381

[8]

Conaway MCP, 2008, LC GC EUR, P14

[9] MALDI-FTICR imaging mass spectrometry of drugs and metabolites in tissue [J].

Cornett, Dale S. ;

Frappier, Sara L. ;

Caprioli, Richard M. .

ANALYTICAL CHEMISTRY, 2008, 80 (14) :5648-5653

[10] Imaging mass spectrometry of neuropeptides in decapod crustacean neuronal tissues [J].

DeKeyser, Stephanie S. ;

Kutz-Naber, Kimberly K. ;

Schmidt, Joshua J. ;

Barrett-Wilt, Gregory A. ;

Li, Lingjun .

JOURNAL OF PROTEOME RESEARCH, 2007, 6 (05) :1782-1791

← 1 2 3 4 →