Proteomic analysis of the carbonate insoluble outer membrane fraction of the soft-rot pathogen Dickeya dadantii (syn. Erwinia chrysanthemi) strain 3937

被引：21

作者：

Babujee, Lavanya ^{[1
]}

Venkatesh, Balakrishnan ^{[1
]}

Yamazaki, Akihiro ^{[1
]}

Tsuyumu, Shinji ^{[1
]}

机构：

[1] Shizuoka Univ, Fac Agr, Plant Pathol Lab, Suruga Ku, Shizuoka 4228529, Japan

来源：

JOURNAL OF PROTEOME RESEARCH | 2007年 / 6卷 / 01期

关键词：

Dickeya dadantii; outer membrane; proteome; virulence factors; plant extract; acidic stress; iron starvation; post-translational modifications;

D O I：

10.1021/pr060423l

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present results of the first comprehensive proteomic analysis of the outer membrane of the bacterial phytopathogen Dickeya dadantii strain 3937 and its response to virulence-contributing factors such as host plant extract, acidic stress, and iron starvation. We analyzed the carbonate-insoluble membrane fractions, which are highly enriched for outer membrane proteins, using two-dimensional electrophoresis and identified the proteins by MALDI-TOF MS. Forty unique proteins were identified, some of which were differentially expressed under the above conditions.

引用

页码：62 / 69

页数：8

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