Use of enzyme-labelled protein G assay for the detection of anti Borrelia burgdorferi antibodies in wild animal sera

被引：9

作者：

Deruaz, D ^{[1
]}

Eid, P ^{[1
]}

Deruaz, J ^{[1
]}

Sempere, A ^{[1
]}

Bourgouin, C ^{[1
]}

Rodhain, F ^{[1
]}

PerezEid, C ^{[1
]}

机构：

[1] INST PASTEUR,UNITE ECOL SYST VECTORIELS,F-75724 PARIS 15,FRANCE

来源：

EUROPEAN JOURNAL OF EPIDEMIOLOGY | 1996年 / 12卷 / 05期

关键词：

Borrelia burgdorferi; IgG antibodies; roe-deer; enzyme-labelled protein G; passive hemagglutination;

D O I：

10.1007/BF00144006

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

A modified ELISA was developed for the detection of anti-Borrelia burgdorferi (Bb) IgG antibodies in wild animal sera based on an Enzyme-Labelled-protein G Assay (ELGA). Microplates were coated with an extract of Bb sensu stricto strain (SV1) as antigen. Specific antibodies of the serum samples were detected by a peroxidase-labelled-protein G. Using comparative immunodiagnosis by means of a passive hemagglutination test (HA), ELGA was tested on 82 roe-deer blood samples,A correlation was found between the two methods (r = 0,66). Good reproducibility of titers was observed by ELGA technique. A minimal crossreactivity was discovered with Leptospira. ELGA could facilitate the recognition of specific antibodies in collections of wild animal sera.

引用

页码：515 / 519

页数：5

共 21 条

[1]

AESCHLIMANN A, 1986, Zentralblatt fuer Bakteriologie Mikrobiologie und Hygiene Series A, V263, P450

[2]

AKERSTROM B, 1985, J IMMUNOL, V135, P2589

[3]

AKERSTROM B, 1986, J BIOL CHEM, V261, P240

[4] DELINEATION OF BORRELIA-BURGDORFERI SENSU-STRICTO, BORRELIA-GARINII SP-NOV, AND GROUP VS461 ASSOCIATED WITH LYME BORRELIOSIS [J].

BARANTON, G ;

POSTIC, D ;

SAINTGIRONS, I ;

BOERLIN, P ;

PIFFARETTI, JC ;

ASSOUS, M ;

GRIMONT, PAD .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1992, 42 (03) :378-383

[5]

BARANTON G, 1989, METHODES LAB LEPTOSP, P107

[6]

BARBOUR AG, 1984, YALE J BIOL MED, V57, P521

[7] STREPTOCOCCAL PROTEIN-G, EXPRESSED BY STREPTOCOCCI OR BY ESCHERICHIA-COLI, HAS SEPARATE BINDING-SITES FOR HUMAN-ALBUMIN AND IGG [J].

BJORCK, L ;

KASTERN, W ;

LINDAHL, G ;

WIDEBACK, K .

MOLECULAR IMMUNOLOGY, 1987, 24 (10) :1113-1122

[8]

DOBY JM, 1991, RECL MED VET, V167, P55

[9]

FORSGREN A, 1966, J IMMUNOL, V97, P822

[10] USE OF STAPHYLOCOCCAL PROTEIN-A AS AN IMMUNOLOGICAL REAGENT [J].

GODING, JW .

JOURNAL OF IMMUNOLOGICAL METHODS, 1978, 20 (APR) :241-253

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