Synaptotagamin I and IV define distinct populations of neuronal transport vesicles

被引:48
作者
Berton, F
Cornet, V
Iborra, C
Garrido, J
Dargent, B
Fukuda, M
Seagar, M
Marquèze, B
机构
[1] Univ Mediterranee, Fac Med, Inst Federat Jean Roche, INSERM,U464, F-13916 Marseille 20, France
[2] Inst Phys & Chem Res, Brain Sci Inst, Dev Neurobiol Lab, Wako, Saitama 3510198, Japan
关键词
development; Golgi; growth cone; rat synaptotagmin; synaptic vesicle;
D O I
10.1046/j.1460-9568.2000.00013.x
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Mammalian synaptotagmins constitute a multigene family of at least 11 membrane proteins. We have characterized synaptotagmin IV using antibodies directed against the C2A domain of the protein. Antibodies reacted specifically with a protein band that migrated as a 41-44 kDa doublet. Synaptotagmin IV expression was regulated throughout development. A strong decrease in the amount detected by Western blotting occurred between postnatal day 5 and adulthood, in agreement with studies on the expression of synaptotagmin IV transcripts. In subcellular fractionation, synaptotagmin IV was not detected in the synaptic vesicle-enriched fraction. Immunofluorescence microscopy was concordant with this finding. In 6-day-old rat cerebellum and cultured hippocampal neurons the subcellular distribution of synaptotagmin IV was clearly different from that of synaptotagmin I. Synaptotagmin IV displayed a punctate non-polarized distribution on neuronal extensions, whereas synaptotagmin I staining was essentially synaptic. Synaptotagmin IV staining was also observed in the some in strong perinuclear fluorescent puncta superimposed on that of Golgi/ TGN markers. Furthermore, synaptotagmin IV was seen in the proximal part of the growth cone domain and not in the microfilament-rich region which includes filopodia. Co-localizations with the adhesion molecules vinculin and zyxin at the proximal part of growth cones were observed. Synaptotagmin IV may thus be involved in the regulation of specific membrane-trafficking pathways during brain development.
引用
收藏
页码:1294 / 1302
页数:9
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