Fluorescent in situ hybridization as a tool to retrospectively identify Cryptosporidium parvum and Giardia lamblia in samples from terrestrial mammalian wildlife

被引:38
作者
Bednarska, Malgorzata
Bajer, Anna
Sinski, Edward
Girouard, Autumn S.
Tamang, Leena
Graczyk, Thaddeus K. [1 ]
机构
[1] Johns Hopkins Bloomberg Sch Publ Hlth, Div Environm Hlth Engn, Dept Environm Hlth Sci, Baltimore, MD 21205 USA
[2] Univ Warsaw, Fac Biol, Inst Zool, Dept Parasitol, PL-00325 Warsaw, Poland
[3] Johns Hopkins Bloomberg Sch Publ Hlth, W Harry Feinstone Dept Mol Microbiol & Immunol, Baltimore, MD 21205 USA
关键词
IMPENETRABLE NATIONAL-PARK; FILTER DISSOLUTION METHOD; WATERBORNE GIARDIASIS; SURFACE WATERS; FECAL SAMPLES; PREVALENCE; OOCYSTS; RODENTS; POLAND; CYSTS;
D O I
10.1007/s00436-006-0276-y
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Fecal samples of five terrestrial mammalian wildlife species stored at 4 degrees C or at -20 degrees C for up to 36 months have been tested for human zoonotic enteric parasites (i.e., Cryptosporidium parvum and Giardia lamblia) using combined fluorescent in situ hybridization (FISH) and direct fluorescent antibody techniques. The prevalence of C. parvum and G. lamblia varied from 20 to 63% (mean, 45.8%) and from 13 to 100% (mean, 53.2%), respectively. The prevalence of C. parvum and G. lamblia infections was higher in small rodents (mean, 68.5%) than in other wildlife (mean, 21%). Overall, 31.1% of animals were coinfected, and coinfections were more prevalent in small rodents (mean, 52%) than in other wildlife species (mean, 13.2%). The present study has shown that the FISH assay can be retrospectively applied to fecal samples for the identification of C. parvum oocysts, but is less suitable for the identification of G. lamblia cysts in such samples. Terrestrial mammalian wildlife, particularly small rodents, can contribute to watershed contamination with C. parvum oocysts and G. lamblia cysts. To control contamination, the management of pristine watersheds used for drinking water purposes should incorporate control measures for terrestrial wildlife, especially field rodents residing within such watersheds.
引用
收藏
页码:455 / 460
页数:6
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