DNA-catalyzed sequence-specific hydrolysis of DNA

被引：144

作者：

Chandra, Madhavaiah ^{[1
]}

Sachdeva, Amit ^{[1
]}

Silverman, Scott K. ^{[1
]}

机构：

[1] Univ Illinois, Dept Chem, Urbana, IL 61801 USA

来源：

NATURE CHEMICAL BIOLOGY | 2009年 / 5卷 / 10期

基金：

美国国家卫生研究院;

关键词：

IN-VITRO SELECTION; ZN2+-DEPENDENT DEOXYRIBOZYMES; STRANDED-DNA; RNA; CLEAVAGE; COMPLEXES; ENZYME; SITE; LINKAGES; RIBOZYME;

D O I：

10.1038/nchembio.201

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Deoxyribozymes (DNA catalysts) have been reported for cleavage of RNA phosphodiester linkages, but cleaving peptide or DNA phosphodiester linkages is much more challenging. Using in vitro selection, here we identified deoxyribozymes that sequence-specifically hydrolyze DNA with multiple turnover and with a rate enhancement of 10(8) (possibly as high as 10(14)). The new DNA catalysts require both Mn2+ and Zn2+, which is noteworthy because many natural DNA nucleases are bimetallic protein enzymes.

引用

收藏

页码：718 / 720

页数：3

相关论文

共 26 条

[1]

Breaker R R, 1994, Chem Biol, V1, P223, DOI 10.1016/1074-5521(94)90014-0

[2] Characterization of a DNA-cleaving deoxyribozyme [J].

Carmi, N ;

Breaker, RR .

BIOORGANIC & MEDICINAL CHEMISTRY, 2001, 9 (10) :2589-2600

[3] CLEAVAGE OF AN AMIDE BOND BY A RIBOZYME [J].

DAI, XC ;

DEMESMAEKER, A ;

JOYCE, GF .

SCIENCE, 1995, 267 (5195) :237-240

[4] Deoxyribozymes with 2′-5′ RNA ligase activity [J].

Flynn-Charlebois, A ;

Wang, YM ;

Prior, TK ;

Rashid, I ;

Hoadley, KA ;

Coppins, RL ;

Wolf, AC ;

Silverman, SK .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (09) :2444-2454

[5] Zn2+-dependent deoxyribozymes that form natural and unnatural RNA linkages [J].

Hoadley, KA ;

Purtha, WE ;

Wolf, AC ;

Flynn-Charlebois, A ;

Silverman, SK .

BIOCHEMISTRY, 2005, 44 (25) :9217-9231

[6] Hybrid restriction enzymes: Zinc finger fusions to Fok I cleavage domain [J].

Kim, YG ;

Cha, J ;

Chandrasegaran, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (03) :1156-1160

[7] Artificial restriction DNA cutter for site-selective scission of double-stranded DNA with tunable scission site and specificity [J].

Komiyama, Makoto ;

Aiba, Yuichiro ;

Yamamoto, Yoji ;

Sumaoka, Jun .

NATURE PROTOCOLS, 2008, 3 (04) :655-662

[8] Controlling the direction of site-selectivity and regioselectivity in RNA ligation by Zn2+-dependent deoxyribozymes that use 2′,3′-cyclic phosphate RNA substrates [J].

Kost, Diana M. ;

Gerdt, Joseph P. ;

Pradeepkumar, P. I. ;

Silverman, Scott K. .

ORGANIC & BIOMOLECULAR CHEMISTRY, 2008, 6 (23) :4391-4398

[9] Site specific self-cleavage of certain assemblies of G-quadruplex [J].

Liu, Xiaoqian ;

Li, Xinming ;

Zhou, Tianyan ;

Wang, Yifan ;

Ng, Magdeline Tao Tao ;

Xu, Wei ;

Li, Tianhu .

CHEMICAL COMMUNICATIONS, 2008, (03) :380-382

[10] Artificial metallonucleases [J].

Mancin, F ;

Scrimin, P ;

Tecilla, P ;

Tonellato, U .

CHEMICAL COMMUNICATIONS, 2005, (20) :2540-2548