The cell wall polymer of the extremely halophilic archaeon Natronococcus occultus

被引:42
作者
Niemetz, R
Karcher, U
Kandler, O
Tindall, BJ
Konig, H
机构
[1] UNIV MUNICH,INST BOT,D-8000 MUNICH,GERMANY
[2] DEUTSCH SAMMLUNG MIKROORGANISMEN & ZELLKULTUREN,D-3300 BRAUNSCHWEIG,GERMANY
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1997年 / 249卷 / 03期
关键词
cell wall; glycoconjugate; poly-(gamma-L-glutamine); Natronococcus; Archaea;
D O I
10.1111/j.1432-1033.1997.00905.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Partial acid hydrolysis of the cell wall polymer produced soluble fragments that could be separated by HPLC. A gamma-glutamyl dipeptide was isolated. In the intact cell wall polymer, the glutamate residues form a poly-(gamma-glutamine) chain with a length of about 60 monomers, which corresponds to a relative molecular mass of approximately 7700 Da. Two other soluble dimeric fragments, composed of glutamate and either glucosamine or galactosamine in a molar ratio of 1:1, were purified from the hydrolysate, suggesting the presence of two different oligosaccharides linked to the poly-(gamma-glutamine) chain of the intact polymer. The analysis of additional fragments, which were composed of an amino sugar and galacturonic acid or glucose indicated that one oligosaccharide consisted of a glucosamine pentamer in an alpha-1,3 linkage at the reducing end and an oligomer with at least five beta-1,4-linked galacturonic acid residues at the non-reducing end. The second oligosaccharide was comprised of a galactosamine dimer in a beta-1,3 linkage at the reducing end and a maltose unit at the non-reducing end. Both oligosaccharides were linked to the alpha-amide group of the glutamine residues of the poly-(gamma-glutamine) chain. The whole cell wall polymer, which represents a novel type of natural glycoconjugate, has a relative molecular mass of 54 kDa.
引用
收藏
页码:905 / 911
页数:7
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