Genomic organization of the human excitatory amino acid transporter gene GLT-1

被引：39

作者：

Meyer, T

Ludolph, AC

Morkel, M

Hagemeier, C

Speer, A

机构：

[1] HUMBOLDT UNIV BERLIN,KLINIKUM CHARITE,LAB PADIAT MOL BIOL,D-10117 BERLIN,GERMANY

[2] UNIV ULM,NEUROL KLIN,D-89081 ULM,GERMANY

[3] TECH FACHHSCH BERLIN,FACHBEREICH CHEM & BIOTECHNOL,D-13347 BERLIN,GERMANY

来源：

NEUROREPORT | 1997年 / 8卷 / 03期

关键词：

coding region; genomic structure; GLT-1; human excitatory amino acid transporter; neurodegenerative disorders;

D O I：

10.1097/00001756-199702100-00039

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

WE present the genomic structure of the human glutamate transporter GLT-1 coding region, the intronic sequences adjacent to the exons, and oligonucleotide primer sequences for single strand conformational analysis. The exon-intron boundaries were determined using long-distance PCR and direct sequencing. The human GLT-1 coding region is composed of 10 exons spanning >50 kb of genomic DNA. The exons range from 127 to 251 bp in length. The intron lengths vary considerably from 2.2 kb to >15 kb. These data provide the basis for implementing a comprehensive screen for genetic alterations in the human GLT-1 gene using genomic DNA as a template.

引用

页码：775 / 777

页数：3

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