Immunogenicity of chimeric multiple antigen peptides based on Plasmodium falciparum antigens: impact of epitope orientation

Assembly of B and T epitopes in multiple antigen peptides (MAP) can bypass genetically predisposed unresponsiveness to B epitopes. Although the underlying mechanisms are unknown, B-cell responses to such diepitope MAP are influenced by intramolecular epitope orientation. In this study, MAP constructs were synthesized, encompassing two epitopes derived from the Plasmodium falciparum antigens circumsporozoite protein (CS) and Pf332. In addition to B epitopes, the sequences comprised T epitopes restricted to mouse H-2(b) (CS) or to H-2(d) and H-2(k) (Pf332) haplotypes. Congenic H-2(b), H-2(d) and H-2(k) Balb mice were immunized with MAP in which the two epitopes were arranged either tandemly or in parallel. Tandemly arranged (B-T)(4) MAP in which the relevant T epitope was positioned adjacent to the lysine core [(Pf332-CS)(4)-core for H-2(b) mice and (CS-Pf332)(4)-core for H-2(d) and H-2(k) mice], elicited the most potent antibody responses in terms of reactivity to both epitopes. Additionally, the (B-T)(4) constructs were generally most efficient in recalling proliferative T-cell responses in vitro, irrespective of the MAP used for in vivo priming. As high antibody titers were generated to both epitopes, the position of B epitopes in the constructs does not appear to be critical for an efficient B-cell response. Rather, the association of strong B-and T-cell responses to the (B-T)(4) MAP constructs suggests that the intramolecular position of the relevant T epitope determines the magnitude of specific antibody production. (C) 1997 Elsevier Science Ltd.